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BMRB: 28112

6YSE

1H, 13C, and 15N chemical shift assignments of the Gp4 from the Pseudomonas phage LUZ24

Authors

Volkov, A.N., Bdira, F.B., Dame, R.T.

Assembly

Gp4 from the Pseudomonas phage LUZ24

Entity

1. Gp4 from the Pseudomonas phage LUZ24 (polymer, Thiol state: not present), 46 monomers, 5654.679 Da Detail

MKSPYEAAHE RALMVNRLQK LTRMLRVHPD PKWKQEQQEL IKRLKK

Formula weight

5654.679 Da

Source organism

Bruynoghevirus LUZ24

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.1 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (587 of 611)	96.0 % (316 of 329)	98.2 % (224 of 228)	87.0 % (47 of 54)
Backbone	98.1 % (265 of 270)	97.8 % (87 of 89)	99.3 % (137 of 138)	95.3 % (41 of 43)
Sidechain	95.1 % (368 of 387)	95.4 % (229 of 240)	97.8 % (133 of 136)	54.5 % (6 of 11)
Aromatic	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (13 of 13)	100.0 % (1 of 1)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. Gp4

MKSPYEAAHE RALMVNRLQK LTRMLRVHPD PKWKQEQQEL IKRLKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

Release date

2020-04-12

Citation

Novel anti-repression mechanism of H-NS proteins by a phage protein
Bdira, F.B., Erkelens, A.M., Qin, L., Volkov, A.N., Lippa, A.M., Bowring, N., Boyle, A.L., Ubbink, M., Dove, S.L., Dame, R.T.
Nucleic Acids Res. (2021), 49, 10770-10784, PubMed 34520554 , DOI 10.1093/nar/gkab793 , Abstract

Related entities 1. Gp4 from the Pseudomonas phage LUZ24, : 1 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

2 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

3 3D HN(CO)CACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

4 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

5 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

7 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

8 3D H(C)CH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

9 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

10 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

12 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

13 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

14 2D (HB)CB(CGCD)HD

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

15 2D (HB)CB(CGCDCE)HE

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Gp4	[U-13C; U-15N]		1 mM
2	Bis-TRIS	natural abundance		20 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	D2O	[U-100% 2H]		6 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr28112_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr28112_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	642		100.0 (chain: 1, length: 46)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 46, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 366
  - ¹³C chemical shifts: 229
  - ¹⁵N chemical shifts: 47
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1