BMRBj - BMREntryMaster

Found 1 Document (2.206 seconds)

BMRB: 30002

5HOU

Solution Structure of p53TAD-TAZ1

Authors

Krois, A.S., Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.

Assembly

Cellular tumor antigen p53,CREB-binding protein (E.C.2.3.1.48)

Entity

1. Cellular tumor antigen p53,CREB-binding protein fusion protein (polymer, Thiol state: free and other bound), 171 monomers, 19086.40 Da Detail

GSHMEEPQSD PSVEPPLSQE TFSDLWKLLP ENNVLSPLPS QAMDDLMLSP DDIEQWFTED PGPDGSCFNG TATGPTADPE KRKLIQQQLV LLLHAHKCQR REQANGEVRA CSLPHCRTMK NVLNHMTHCQ AGKACQVAHC ASSRQIISHW KNCTRHDCPV CLPLKNASDK R

2. ZINC ION (non-polymer), 65.409 × 3 Da

Total weight

19282.627 Da

Max. entity weight

19086.4 Da

Entity Connection

na 9 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS98:SG	2:ZN1:ZN
2	na	sing	1:CYS111:SG	2:ZN1:ZN
3	na	sing	1:CYS116:SG	2:ZN1:ZN
4	na	sing	1:CYS129:SG	2:ZN1:ZN
5	na	sing	1:CYS135:SG	2:ZN1:ZN
6	na	sing	1:CYS140:SG	2:ZN1:ZN
7	na	sing	1:CYS153:SG	2:ZN1:ZN
8	na	sing	1:CYS158:SG	2:ZN1:ZN
9	na	sing	1:CYS161:SG	2:ZN1:ZN

Source organism

Homo sapiens , Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.0 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.0 % (1715 of 1948)	95.7 % (981 of 1025)	76.1 % (566 of 744)	93.9 % (168 of 179)
Backbone	82.8 % (825 of 996)	96.7 % (323 of 334)	70.2 % (355 of 506)	94.2 % (147 of 156)
Sidechain	93.6 % (1045 of 1116)	95.4 % (659 of 691)	90.8 % (365 of 402)	91.3 % (21 of 23)
Aromatic	89.2 % (91 of 102)	98.0 % (50 of 51)	79.2 % (38 of 48)	100.0 % (3 of 3)
Methyl	96.7 % (145 of 150)	97.3 % (73 of 75)	96.0 % (72 of 75)

1. Cellular tumor antigen p53,CREB-binding protein fusion protein

GSHMEEPQSD PSVEPPLSQE TFSDLWKLLP ENNVLSPLPS QAMDDLMLSP DDIEQWFTED PGPDGSCFNG TATGPTADPE KRKLIQQQLV LLLHAHKCQR REQANGEVRA CSLPHCRTMK NVLNHMTHCQ AGKACQVAHC ASSRQIISHW KNCTRHDCPV CLPLKNASDK R

Show sample condition

Sample #1

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [U-15N] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	D2O	[U-2H]	5 %
2	DTT	natural abundance	2 mM
3	TRIS	natural abundance	20 mM
4	p53TAD-TAZ1 fusion protein	[U-15N] p53TAD - [NA] TAZ1	1.0 mM
5	sodium chloride	natural abundance	50 mM
6	H2O	[U-2H]	95 %

Sample #2

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [U-15N][U-13C] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
7	D2O	[U-2H]	99 %
8	DTT	[U-2H]	2 mM
9	TRIS	[U-2H]	20 mM
10	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
11	sodium chloride	natural abundance	50 mM
12	H2O	[U-2H]	1 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [NA] p53TAD - [U-15N] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
13	D2O	[U-2H]	5 %
14	DTT	natural abundance	2 mM
15	TRIS	natural abundance	20 mM
16	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N] TAZ1	1.0 mM
17	sodium chloride	natural abundance	50 mM
18	H2O	[U-2H]	95 %

Sample #4

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
19	D2O	[U-2H]	99 %
20	DTT	[U-2H]	2 mM
21	TRIS	[U-2H]	20 mM
22	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1.0 mM
23	sodium chloride	natural abundance	50 mM
24	D2O	[U-2H]	1 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [U-15N][U-13C] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5HOU, Strand ID: A Detail

Release date

2016-03-13

Citation

Recognition of the disordered p53 transactivation domain by the transcriptional adapter zinc finger domains of CREB-binding protein
Krois, A.S., Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.
Proc. Natl. Acad. Sci. U. S. A. (2016), 113, E1853-E1862, PubMed 26976603 , DOI 10.1073/pnas.1602487113 , Abstract

Related entities 1. Cellular tumor antigen p53,CREB-binding protein fusion protein, : 1 : 1 entities Detail

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

6 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

7 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

8 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.4, Details 1.0 mM [U-15N][U-13C] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

10 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.4, Details 1 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

nullKeywords TRANSFERASE, binding motif, intrinsically disordered protein, protein-protein interaction, transcriptional coactivator, tumor suppressor

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Found 1 Document (2.206 seconds)

BMRB: 30002

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Sample #6

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Cellular tumor antigen p53,CREB-binding protein fusion protein, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

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