BMRBj - BMREntryMaster

Found 1 Document (1.047 seconds)

BMRB: 30002

5HOU

Solution Structure of p53TAD-TAZ1

Authors

Krois, A.S., Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.

Assembly

Cellular tumor antigen p53,CREB-binding protein (E.C.2.3.1.48)

Entity

1. Cellular tumor antigen p53,CREB-binding protein fusion protein (polymer, Thiol state: free and other bound), 171 monomers, 19086.40 Da Detail

GSHMEEPQSD PSVEPPLSQE TFSDLWKLLP ENNVLSPLPS QAMDDLMLSP DDIEQWFTED PGPDGSCFNG TATGPTADPE KRKLIQQQLV LLLHAHKCQR REQANGEVRA CSLPHCRTMK NVLNHMTHCQ AGKACQVAHC ASSRQIISHW KNCTRHDCPV CLPLKNASDK R

2. ZINC ION (non-polymer), 65.409 × 3 Da

Total weight

19282.627 Da

Max. entity weight

19086.4 Da

Entity Connection

na 9 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS98:SG	2:ZN1:ZN
2	na	sing	1:CYS111:SG	2:ZN1:ZN
3	na	sing	1:CYS116:SG	2:ZN1:ZN
4	na	sing	1:CYS129:SG	2:ZN1:ZN
5	na	sing	1:CYS135:SG	2:ZN1:ZN
6	na	sing	1:CYS140:SG	2:ZN1:ZN
7	na	sing	1:CYS153:SG	2:ZN1:ZN
8	na	sing	1:CYS158:SG	2:ZN1:ZN
9	na	sing	1:CYS161:SG	2:ZN1:ZN

Source organism

Homo sapiens , Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.0 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.0 % (1715 of 1948)	95.7 % (981 of 1025)	76.1 % (566 of 744)	93.9 % (168 of 179)
Backbone	82.8 % (825 of 996)	96.7 % (323 of 334)	70.2 % (355 of 506)	94.2 % (147 of 156)
Sidechain	93.6 % (1045 of 1116)	95.4 % (659 of 691)	90.8 % (365 of 402)	91.3 % (21 of 23)
Aromatic	89.2 % (91 of 102)	98.0 % (50 of 51)	79.2 % (38 of 48)	100.0 % (3 of 3)
Methyl	96.7 % (145 of 150)	97.3 % (73 of 75)	96.0 % (72 of 75)

1. Cellular tumor antigen p53,CREB-binding protein fusion protein

GSHMEEPQSD PSVEPPLSQE TFSDLWKLLP ENNVLSPLPS QAMDDLMLSP DDIEQWFTED PGPDGSCFNG TATGPTADPE KRKLIQQQLV LLLHAHKCQR REQANGEVRA CSLPHCRTMK NVLNHMTHCQ AGKACQVAHC ASSRQIISHW KNCTRHDCPV CLPLKNASDK R

Show sample condition

Sample #1

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [U-15N] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	D2O	[U-2H]	5 %
2	DTT	natural abundance	2 mM
3	TRIS	natural abundance	20 mM
4	p53TAD-TAZ1 fusion protein	[U-15N] p53TAD - [NA] TAZ1	1.0 mM
5	sodium chloride	natural abundance	50 mM
6	H2O	[U-2H]	95 %

Sample #2

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [U-15N][U-13C] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
7	D2O	[U-2H]	99 %
8	DTT	[U-2H]	2 mM
9	TRIS	[U-2H]	20 mM
10	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
11	sodium chloride	natural abundance	50 mM
12	H2O	[U-2H]	1 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [NA] p53TAD - [U-15N] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
13	D2O	[U-2H]	5 %
14	DTT	natural abundance	2 mM
15	TRIS	natural abundance	20 mM
16	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N] TAZ1	1.0 mM
17	sodium chloride	natural abundance	50 mM
18	H2O	[U-2H]	95 %

Sample #4

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
19	D2O	[U-2H]	99 %
20	DTT	[U-2H]	2 mM
21	TRIS	[U-2H]	20 mM
22	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1.0 mM
23	sodium chloride	natural abundance	50 mM
24	D2O	[U-2H]	1 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1.0 mM [U-15N][U-13C] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5HOU, Strand ID: A Detail

Release date

2016-03-13

Citation

Recognition of the disordered p53 transactivation domain by the transcriptional adapter zinc finger domains of CREB-binding protein
Krois, A.S., Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.
Proc. Natl. Acad. Sci. U. S. A. (2016), 113, E1853-E1862, PubMed 26976603 , DOI 10.1073/pnas.1602487113 , Abstract

Related entities 1. Cellular tumor antigen p53,CREB-binding protein fusion protein, : 1 : 1 entities Detail

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

6 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

7 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 1 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

8 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.4, Details 1.0 mM [U-15N][U-13C] p53TAD - [NA] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
25	D2O	[U-2H]	5 %
26	DTT	natural abundance	2 mM
27	TRIS	natural abundance	20 mM
28	p53TAD-TAZ1 fusion protein	[U-15N][U-13C] p53TAD - [NA] TAZ1	1.0 mM
29	sodium chloride	natural abundance	50 mM
30	H2O	[U-2H]	95 %

10 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.4, Details 1 mM [NA] p53TAD - [U-15N][U-13C] TAZ1 p53TAD-TAZ1 fusion protein, 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	D2O	[U-2H]	5 %
32	DTT	natural abundance	2 mM
33	TRIS	natural abundance	20 mM
34	p53TAD-TAZ1 fusion protein	[NA] p53TAD - [U-15N][U-13C] TAZ1	1 mM
35	sodium chloride	natural abundance	50 mM
36	H2O	[U-2H]	95 %

nullKeywords TRANSFERASE, binding motif, intrinsically disordered protein, protein-protein interaction, transcriptional coactivator, tumor suppressor

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.047 seconds)

BMRB: 30002

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Sample #6

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Cellular tumor antigen p53,CREB-binding protein fusion protein, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample