BMRBj - BMREntryMaster

Found 1 Document (5.858 seconds)

BMRB: 30004

5HPD

Solution Structure of TAZ2-p53TAD

Authors

Krois, A.S., Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.

Assembly

CREB-binding protein,Cellular tumor antigen p53 (E.C.2.3.1.48)

Entity

1. CREB-binding protein,Cellular tumor antigen p53 fusion protein (polymer, Thiol state: free and other bound), 158 monomers, 17688.03 Da Detail

SPQESRRLSI QRCIQSLVHA CQCRNANCSL PSCQKMKRVV QHTKGCKRKT NGGCPVCKQL IALCCYHAKH CQENKCPVPF CLNIKHKLRQ QQGSGSGSEE PQSDPSVEPP LSQETFSDLW KLLPENNVLS PLPSQAMDDL MLSPDDIEQW FTEDPGPD

2. ZINC ION (non-polymer), 65.409 × 3 Da

Total weight

17884.256 Da

Max. entity weight

17688.03 Da

Entity Connection

na 9 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS23:SG	2:ZN1:ZN
2	na	sing	1:CYS28:SG	2:ZN1:ZN
3	na	sing	1:CYS33:SG	2:ZN1:ZN
4	na	sing	1:CYS46:SG	2:ZN1:ZN
5	na	sing	1:CYS54:SG	2:ZN1:ZN
6	na	sing	1:CYS57:SG	2:ZN1:ZN
7	na	sing	1:CYS71:SG	2:ZN1:ZN
8	na	sing	1:CYS76:SG	2:ZN1:ZN
9	na	sing	1:CYS81:SG	2:ZN1:ZN

Source organism

Mus musculus , Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.9 %, Completeness (bb): 80.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.9 % (1629 of 1833)	97.2 % (948 of 975)	76.3 % (527 of 691)	92.2 % (154 of 167)
Backbone	80.7 % (741 of 918)	95.8 % (295 of 308)	67.5 % (315 of 467)	91.6 % (131 of 143)
Sidechain	96.9 % (1033 of 1066)	97.9 % (653 of 667)	95.2 % (357 of 375)	95.8 % (23 of 24)
Aromatic	89.0 % (73 of 82)	97.6 % (40 of 41)	79.5 % (31 of 39)	100.0 % (2 of 2)
Methyl	98.4 % (124 of 126)	98.4 % (62 of 63)	98.4 % (62 of 63)

1. CREB-binding protein,Cellular tumor antigen p53 fusion protein

SPQESRRLSI QRCIQSLVHA CQCRNANCSL PSCQKMKRVV QHTKGCKRKT NGGCPVCKQL IALCCYHAKH CQENKCPVPF CLNIKHKLRQ QQGSGSGSEE PQSDPSVEPP LSQETFSDLW KLLPENNVLS PLPSQAMDDL MLSPDDIEQW FTEDPGPD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1.75 mM [U-13C; U-15N] TAZ2-p53TAD, 20 mM TRIS, 50 mM sodium chloride, 1 mM DTT, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	D2O	[U-2H]	10 %
2	DTT	natural abundance	1 mM
3	TAZ2-p53TAD	[U-13C; U-15N]	1.75 (±0.25) mM
4	TRIS	natural abundance	20 mM
5	sodium chloride	natural abundance	50 mM
6	H2O	[U-2H]	90 %

Sample #2

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1.75 mM [U-13C; U-15N] TAZ2-p53TAD, 20 mM [U-2H] TRIS, 50 mM sodium chloride, 1 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
7	D2O	[U-2H]	99 %
8	DTT	[U-2H]	1 mM
9	TAZ2-p53TAD	[U-13C; U-15N]	1.75 (±0.25) mM
10	TRIS	[U-2H]	20 mM
11	sodium chloride	natural abundance	50 mM
12	H2O	[U-2H]	1 %

Sample #3

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM [U-13C] TAZ2, 1 mM p53(13-37, 1 mM p53(38-61), 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 20 mM [U-2H] TRIS, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
13	D2O	[U-2H]	99 %
14	DTT	[U-2H]	2 mM
15	TAZ2	[U-13C]	1 mM
16	TRIS	[U-2H]	20 mM
17	p53(13-37	natural abundance	1 mM
18	p53(38-61)	natural abundance	1 mM
19	sodium chloride	natural abundance	50 mM
20	H2O	[U-2H]	1 %

Sample #4

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM TAZ2, 1 mM [U-13C] p53(13-37), 1 mM [U-13C] p53(38-61), 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
21	D2O	[U-2H]	99 %
22	DTT	[U-2H]	2 mM
23	TAZ2	natural abundance	1 mM
24	TRIS	[U-2H]	20 mM
25	p53(13-37)	[U-13C]	1 mM
26	p53(38-61)	[U-13C]	1 mM
27	sodium chloride	natural abundance	50 mM
28	H2O	[U-2H]	1 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM TAZ2, 1 mM [U-15N] p53(13-61), 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
29	D2O	[U-2H]	10 %
30	DTT	natural abundance	2 mM
31	TAZ2	natural abundance	1 mM
32	TRIS	natural abundance	20 mM
33	p53(13-61)	[U-15N]	1 mM
34	sodium chloride	natural abundance	50 mM
35	H2O	[U-2H]	90 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM [U-15N] TAZ2, 1 mM p53(13-61), 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
36	D2O	[U-2H]	10 %
37	DTT	natural abundance	2 mM
38	TAZ2	[U-15N]	1 mM
39	TRIS	natural abundance	20 mM
40	p53(13-61)	natural abundance	1 mM
41	sodium chloride	natural abundance	50 mM
42	H2O	[U-2H]	90 %

Sample #7

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM TAZ2, 1 mM [U-13C] p53(13-61), 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
43	D2O	[U-2H]	99 %
44	DTT	[U-2H]	2 mM
45	TAZ2	natural abundance	1 mM
46	TRIS	[U-2H]	20 mM
47	p53(13-61)	[U-13C]	1 mM
48	sodium chloride	natural abundance	50 mM
49	H2O	[U-2H]	1 %

Sample #8

Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM [U-13C] TAZ2, 1 mM p53(13-61), 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
50	D2O	[U-2H]	99 %
51	DTT	[U-2H]	2 mM
52	TAZ2	[U-13C]	1 mM
53	TRIS	[U-2H]	20 mM
54	p53(13-61)	natural abundance	1 mM
55	sodium chloride	natural abundance	50 mM
56	H2O	[U-2H]	1 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5HPD, Strand ID: A Detail

Release date

2016-03-13

Citation

Recognition of the disordered p53 transactivation domain by the transcriptional adapter zinc finger domains of CREB-binding protein
Krois, A.S., Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.
Proc. Natl. Acad. Sci. U. S. A. (2016), 113, E1853-E1862, PubMed 26976603 , DOI 10.1073/pnas.1602487113 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30002 released on 2016-03-13
    Title Solution Structure of p53TAD-TAZ1
  BMRB: 30003 released on 2016-03-13
    Title Solution Structure of TAZ2-p53AD2

Related entities 1. CREB-binding protein,Cellular tumor antigen p53 fusion protein, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1.75 mM [U-13C; U-15N] TAZ2-p53TAD, 20 mM TRIS, 50 mM sodium chloride, 1 mM DTT, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	D2O	[U-2H]	10 %
2	DTT	natural abundance	1 mM
3	TAZ2-p53TAD	[U-13C; U-15N]	1.75 (±0.25) mM
4	TRIS	natural abundance	20 mM
5	sodium chloride	natural abundance	50 mM
6	H2O	[U-2H]	90 %

2 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.4, Details 1.75 mM [U-13C; U-15N] TAZ2-p53TAD, 20 mM [U-2H] TRIS, 50 mM sodium chloride, 1 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
7	D2O	[U-2H]	99 %
8	DTT	[U-2H]	1 mM
9	TAZ2-p53TAD	[U-13C; U-15N]	1.75 (±0.25) mM
10	TRIS	[U-2H]	20 mM
11	sodium chloride	natural abundance	50 mM
12	H2O	[U-2H]	1 %

3 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	[U-2H]	10 %
2	DTT	natural abundance	1 mM
3	TAZ2-p53TAD	[U-13C; U-15N]	1.75 (±0.25) mM
4	TRIS	natural abundance	20 mM
5	sodium chloride	natural abundance	50 mM
6	H2O	[U-2H]	90 %

4 3D 1H-15N TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM TAZ2, 1 mM [U-15N] p53(13-61), 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
29	D2O	[U-2H]	10 %
30	DTT	natural abundance	2 mM
31	TAZ2	natural abundance	1 mM
32	TRIS	natural abundance	20 mM
33	p53(13-61)	[U-15N]	1 mM
34	sodium chloride	natural abundance	50 mM
35	H2O	[U-2H]	90 %

5 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.4, Details 1 mM TAZ2, 1 mM [U-13C] p53(13-61), 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
43	D2O	[U-2H]	99 %
44	DTT	[U-2H]	2 mM
45	TAZ2	natural abundance	1 mM
46	TRIS	[U-2H]	20 mM
47	p53(13-61)	[U-13C]	1 mM
48	sodium chloride	natural abundance	50 mM
49	H2O	[U-2H]	1 %

6 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
29	D2O	[U-2H]	10 %
30	DTT	natural abundance	2 mM
31	TAZ2	natural abundance	1 mM
32	TRIS	natural abundance	20 mM
33	p53(13-61)	[U-15N]	1 mM
34	sodium chloride	natural abundance	50 mM
35	H2O	[U-2H]	90 %

7 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.4, Details 1 mM [U-13C] TAZ2, 1 mM p53(13-61), 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
50	D2O	[U-2H]	99 %
51	DTT	[U-2H]	2 mM
52	TAZ2	[U-13C]	1 mM
53	TRIS	[U-2H]	20 mM
54	p53(13-61)	natural abundance	1 mM
55	sodium chloride	natural abundance	50 mM
56	H2O	[U-2H]	1 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8, Details 1 mM [U-15N] TAZ2, 1 mM p53(13-61), 20 mM TRIS, 50 mM sodium chloride, 2 mM DTT, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
36	D2O	[U-2H]	10 %
37	DTT	natural abundance	2 mM
38	TAZ2	[U-15N]	1 mM
39	TRIS	natural abundance	20 mM
40	p53(13-61)	natural abundance	1 mM
41	sodium chloride	natural abundance	50 mM
42	H2O	[U-2H]	90 %

9 3D HCCH-COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.4, Details 1 mM [U-13C] TAZ2, 1 mM p53(13-37, 1 mM p53(38-61), 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 20 mM [U-2H] TRIS, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
13	D2O	[U-2H]	99 %
14	DTT	[U-2H]	2 mM
15	TAZ2	[U-13C]	1 mM
16	TRIS	[U-2H]	20 mM
17	p53(13-37	natural abundance	1 mM
18	p53(38-61)	natural abundance	1 mM
19	sodium chloride	natural abundance	50 mM
20	H2O	[U-2H]	1 %

10 3D HCCH-COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 99% D2O, 1% H2O, Pressure 1 atm, Temperature 305 K, pH 6.4, Details 1 mM TAZ2, 1 mM [U-13C] p53(13-37), 1 mM [U-13C] p53(38-61), 20 mM [U-2H] TRIS, 50 mM sodium chloride, 2 mM [U-2H] DTT, 99 % [U-2H] D2O, 99% D2O, 1% H2O

#	Name	Isotope labeling	Concentration
21	D2O	[U-2H]	99 %
22	DTT	[U-2H]	2 mM
23	TAZ2	natural abundance	1 mM
24	TRIS	[U-2H]	20 mM
25	p53(13-37)	[U-13C]	1 mM
26	p53(38-61)	[U-13C]	1 mM
27	sodium chloride	natural abundance	50 mM
28	H2O	[U-2H]	1 %

nullKeywords TRANSFERASE, binding motif, intrinsically disordered protein, protein-protein interaction, transcriptional coactivator, tumor suppressor

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (5.858 seconds)

BMRB: 30004

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Sample #6

Sample #7

Sample #8

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. CREB-binding protein,Cellular tumor antigen p53 fusion protein, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample