BMRBj - BMREntryMaster

Found 1 Document (5.033 seconds)

BMRB: 30005

5HUZ

Solution structure of coiled coil domain of myosin binding subunit of myosin light chain phosphatase

Authors

Sharma, A.K., Birrane, G., Anklin, C., Rigby, A.C., Pollak, M., Alper, S.L.

Assembly

Protein phosphatase 1 regulatory subunit 12A

Entity

1. Protein phosphatase 1 regulatory subunit 12A (polymer, Thiol state: not present), 47 monomers, 5663.373 × 2 Da Detail

DFKKLYEQIL AENEKLKAQL HDTNMELTDL KLQLEKATQR QERFARS

Total weight

11326.746 Da

Max. entity weight

5663.373 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.1 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (554 of 595)	94.0 % (298 of 317)	92.0 % (206 of 224)	92.6 % (50 of 54)
Backbone	97.2 % (274 of 282)	96.8 % (91 of 94)	97.2 % (137 of 141)	97.9 % (46 of 47)
Sidechain	90.8 % (327 of 360)	92.8 % (207 of 223)	89.2 % (116 of 130)	57.1 % (4 of 7)
Aromatic	53.1 % (17 of 32)	68.8 % (11 of 16)	37.5 % (6 of 16)
Methyl	98.0 % (49 of 50)	96.0 % (24 of 25)	100.0 % (25 of 25)

1. entity 1

DFKKLYEQIL AENEKLKAQL HDTNMELTDL KLQLEKATQR QERFARS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 7.0 (±0.05), Details 1.0 mM [U-99% 13C; U-99% 15N] coiled coil mbs, 1.0 mM [U-99% 15N] coiled coil mbs, 1.0 mM coiled coil mbs, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 5HUZ, Strand ID: A, B Detail

Release date

2016-03-13

Citation

NMR assignment and secondary structure of coiled coil domain of C-terminal myosin binding subunit of myosin phosphatase
Sharma, A.K., Rigby, A.C., Sharma, A.K., Rigby, A.C.
Protein Pept. Lett. (2014), 21, 639-645, PubMed 24693955 , DOI: , Abstract

Related entities 1. Protein phosphatase 1 regulatory subunit 12A, : 1 : 7 : 23 entities Detail

Interaction partners 1. Protein phosphatase 1 regulatory subunit 12A, : 9 interactors Detail

More details for 9 interactors identified by 7 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 7.0 (±0.05), Details 1.0 mM [U-99% 13C; U-99% 15N] coiled coil mbs, 1.0 mM [U-99% 15N] coiled coil mbs, 1.0 mM coiled coil mbs, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

2 2D 1H-15N HSQC NH2 ONLY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

3 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

5 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

6 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

7 3D HBHA(CO) NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

8 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

9 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

10 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

11 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

12 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

13 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

14 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

15 2D 1H-1H NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

16 isotope filtered 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 7.0 (±0.05), Details 1.0 mM [U-99% 13C; U-99% 15N] coiled coil mbs, 1.0 mM [U-99% 15N] coiled coil mbs, 1.0 mM coiled coil mbs, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

17 isotope filtered 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

18 isotope filtered 2D NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

19 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	coiled coil mbs-1	[U-99% 13C; U-99% 15N]	1.0 mM
2	coiled coil mbs-2	[U-99% 15N]	1.0 mM
3	coiled coil mbs-3	natural abundance	1.0 mM

nullKeywords SIGNALING PROTEIN, VASCULAR SMOOTH MUSCLE CELL

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Found 1 Document (5.033 seconds)

BMRB: 30005

Sample

Related entities 1. Protein phosphatase 1 regulatory subunit 12A, : 1 : 7 : 23 entities Detail

Interaction partners 1. Protein phosphatase 1 regulatory subunit 12A, : 9 interactors Detail

Experiments performed 19 experiments Detail

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