BMRB: 30016

DNA duplex containing a ribonolactone lesion

Authors

Zalesak, J., Constant, J.F., Jourdan, M.

Assembly

DNA (5'-D(*CP*GP*CP*TP*CP*(RIB)P*CP*AP*CP*GP*C)-3'), DNA (5'-D(*GP*CP*(8OG)P*TP*GP*GP*GP*AP*GP*CP*G)-3')

Entity

1. DNA (5'-D(*CP*GP*CP*TP*CP*(RIB)P*CP*AP*CP*GP*C)-3'), DNA (5'-D(*GP*CP*(8OG)P*TP*GP*GP*GP*AP*GP*CP*G)-3'), entity 1 (polymer, Thiol state: not present), 11 monomers, 3222.996 Da Detail

CGCTCXCACG C

2. DNA (5'-D(*CP*GP*CP*TP*CP*(RIB)P*CP*AP*CP*GP*C)-3'), DNA (5'-D(*GP*CP*(8OG)P*TP*GP*GP*GP*AP*GP*CP*G)-3'), entity 2 (polymer, Thiol state: not present), 11 monomers, 3534.218 Da Detail

GCXTGGGAGC G

Total weight

6757.214 Da

Max. entity weight

3534.218 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 86.9 %, Completeness (bb): 82.1 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	86.9 % (172 of 198)	86.9 % (172 of 198)
Suger, PO4	82.1 % (115 of 140)	82.1 % (115 of 140)
Nucleobase	98.3 % (57 of 58)	98.3 % (57 of 58)
Aromatic	97.5 % (39 of 40)	97.5 % (39 of 40)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. entity 1

CGCTCXCACG C

2. entity 2

GCXTGGGAGC G

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 bar, Temperature 283 K, pH 6, Details 0.85 mM oligonucleotide, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	none		0.85 mM
2	entity_2	none		0.85 mM
3	NaCl	natural abundance		100 mM
4	sodium phosphate buffer	natural abundance		20 mM

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Release date

2016-09-08

Citation

NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions
Zalesak, J., Constant, J.F., Jourdan, M.
Biochemistry (2016), 55, 3899-3906, PubMed 27322640 , DOI 10.1021/acs.biochem.6b00396 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 3 experiments Detail