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BMRB: 30023

5IEB

Solution structure of SdrG from Sphingomonas melonis Fr1

Authors

Campagne, S., Vorholt, J.A., Allain, F.

Assembly

Sensory transduction regulatory protein

Entity

1. Sensory transduction regulatory protein (polymer), 130 monomers, 13875.64 Da Detail

MSALTQILIV EDEPLIAMML EDFLEVLDKT PVGTVDTVAG ALARVEDGGI DAAILDVNLR GGEKSTPVAE ALAARDIPFV FATGGSDDSV DSRFRDRPVL QKPFTMDGVA KALAALLVPR GSVEHHHHHH

Formula weight

13875.64 Da

Source organism

Sphingomonas melonis FR1

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 91.3 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1295 of 1419)	92.1 % (666 of 723)	89.3 % (509 of 570)	95.2 % (120 of 126)
Backbone	93.0 % (712 of 766)	93.9 % (247 of 263)	91.6 % (348 of 380)	95.1 % (117 of 123)
Sidechain	90.3 % (698 of 773)	91.1 % (419 of 460)	89.0 % (276 of 310)	100.0 % (3 of 3)
Aromatic	47.3 % (35 of 74)	62.2 % (23 of 37)	32.4 % (12 of 37)
Methyl	98.4 % (179 of 182)	97.8 % (89 of 91)	98.9 % (90 of 91)

1. Sensory transduction regulatory protein

MSALTQILIV EDEPLIAMML EDFLEVLDKT PVGTVDTVAG ALARVEDGGI DAAILDVNLR GGEKSTPVAE ALAARDIPFV FATGGSDDSV DSRFRDRPVL QKPFTMDGVA KALAALLVPR GSVEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5IEB, Strand ID: A Detail

Release date

2016-07-12

Citation

Role of the PFXFATG[G/Y] Motif in the Activation of SdrG, a Response Regulator Involved in the Alphaproteobacterial General Stress Response
Campagne, S., Dintner, S., Gottschlich, L., Thibault, M., Bortfeld-Miller, M., Kaczmarczyk, A., Francez-Charlot, A., Allain, F.H.-T., Vorholt, J.A.
Structure (2016), 24, 1237-1247, PubMed 27396826 , DOI 10.1016/j.str.2016.05.015 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30025 released on 2016-07-12
Title Solution structure of the BeF3-activated conformation of SdrG from Pseudomonas melonis Fr1

Related entities 1. Sensory transduction regulatory protein, : 1 : 1 : 18 entities Detail

Experiments performed 10 experiments Detail

1 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceII - 900 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

2 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceII - 900 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

3 3D 1H-15N NOESY

Instrument

Bruker AvanceII - 900 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

4 3D HNCO

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

5 3D HNCACB

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

6 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

7 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

8 3D C(CO)NH

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

9 3D H(CCO)NH

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

10 1D H

Instrument

Bruker AvanceIII - 500 MHz cryo probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] SdrG, 10 mM NaPO4, 50 mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaCl	natural abundance		50 mM
2	NaPO4	natural abundance		10 mM
3	SdrG	[U-99% 13C; U-99% 15N]		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

nullKeywords PROTEIN, Single domain response regulator FAT GUY Familly