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Found 1 Document (0.911 seconds)

BMRB: 30025

5IEJ

Solution structure of the BeF3-activated conformation of SdrG from Pseudomonas melonis Fr1

Authors

Campagne, S., Vorholt, J.A., Allain, F.

Assembly

SdrG

Entity

1. SdrG (polymer, Thiol state: not present), 130 monomers, 13875.64 Da Detail

MSALTQILIV EDEPLIAMML EDFLEVLDKT PVGTVDTVAG ALARVEDGGI DAAILDVNLR GGEKSTPVAE ALAARDIPFV FATGGSDDSV DSRFRDRPVL QKPFTMDGVA KALAALLVPR GSVEHHHHHH

Formula weight

13875.64 Da

Source organism

Sphingomonas melonis FR1

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 90.8 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.8 % (1289 of 1419)	92.1 % (666 of 723)	88.4 % (504 of 570)	94.4 % (119 of 126)
Backbone	92.0 % (705 of 766)	93.5 % (246 of 263)	90.3 % (343 of 380)	94.3 % (116 of 123)
Sidechain	90.2 % (697 of 773)	91.3 % (420 of 460)	88.4 % (274 of 310)	100.0 % (3 of 3)
Aromatic	51.4 % (38 of 74)	64.9 % (24 of 37)	37.8 % (14 of 37)
Methyl	99.5 % (181 of 182)	100.0 % (91 of 91)	98.9 % (90 of 91)

1. SdrG

MSALTQILIV EDEPLIAMML EDFLEVLDKT PVGTVDTVAG ALARVEDGGI DAAILDVNLR GGEKSTPVAE ALAARDIPFV FATGGSDDSV DSRFRDRPVL QKPFTMDGVA KALAALLVPR GSVEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] BeF3-activated conformation of SdrG, 10 mM sodium phosphate, 50 mM sodium chloride, 5 mM beryllium Chloride, 30 mM sodium fluoride, 10 mM magnesium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.8 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	0.09 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	0.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.8 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	0.09 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	0.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.8 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	0.09 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	0.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.8 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	0.09 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	0.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5IEJ, Strand ID: A Detail

Release date

2016-07-12

Citation

Role of the PFXFATG[G/Y] Motif in the Activation of SdrG, a Response Regulator Involved in the Alphaproteobacterial General Stress Response
Campagne, S., Dintner, S., Gottschlich, L., Thibault, M., Bortfeld-Miller, M., Kaczmarczyk, A., Francez-Charlot, A., Allain, F.H.-T., Vorholt, J.A.
Structure (2016), 24, 1237-1247, PubMed 27396826 , DOI 10.1016/j.str.2016.05.015 , Abstract

Related entities 1. SdrG, : 1 : 1 : 18 entities Detail

Experiments performed 9 experiments Detail

1 1D H

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] BeF3-activated conformation of SdrG, 10 mM sodium phosphate, 50 mM sodium chloride, 5 mM beryllium Chloride, 30 mM sodium fluoride, 10 mM magnesium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D H(CCO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 3D C(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

7 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

8 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	BeF3-activated conformation of SdrG	[U-99% 13C; U-99% 15N]	1 mM
2	beryllium Chloride	natural abundance	5 mM
3	magnesium chloride	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	sodium fluoride	natural abundance	30 mM
6	sodium phosphate	natural abundance	10 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

nullKeywords PROTEIN, Single domain response regulator FAT GUY response regulator Allosteric transition General stress response

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Found 1 Document (0.911 seconds)

BMRB: 30025

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SdrG, : 1 : 1 : 18 entities Detail

Experiments performed 9 experiments Detail

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