BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	74.1 % (238 of 321)	90.8 % (148 of 163)	54.0 % (68 of 126)	68.8 % (22 of 32)
Backbone	65.5 % (114 of 174)	93.2 % (55 of 59)	44.2 % (38 of 86)	72.4 % (21 of 29)
Sidechain	83.4 % (146 of 175)	89.4 % (93 of 104)	76.5 % (52 of 68)	33.3 % (1 of 3)
Aromatic	80.0 % (24 of 30)	86.7 % (13 of 15)	73.3 % (11 of 15)
Methyl	79.4 % (27 of 34)	88.2 % (15 of 17)	70.6 % (12 of 17)

1. entity 1

HILYACDSCG DKFLDANSLA QHVRIHTAQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.75 mM 1H ZF 13, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ZF 13	natural abundance	0.75 mM
2	ZnCl2	natural abundance	2 mM
3	KCl	natural abundance	50 mM
4	Bis Tris	[U-2H]	10 mM
5	TECP	natural abundance	2 mM

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Calculated from 20 models in PDB: 5ION, Strand ID: A Detail

Release date

2016-03-27

Citation

Solution structure of the 13th C2H2 Zinc Finger of Miz-1
Tremblay, C., Bedard, M., Bonin, M., Lavigne, P.
Biochem. Biophys. Res. Commun. (2016), 473, 471-475, PubMed 26972249 , DOI 10.1016/j.bbrc.2016.03.034 , Abstract

Related entities 1. Zinc finger and BTB domain-containing protein 17, entity 1, : 1 : 5 : 1302 entities Detail

Interaction partners 1. Zinc finger and BTB domain-containing protein 17, entity 1, : 21 interactors Detail

More details for 21 interactors identified by 12 citations

Experiments performed 5 experiments Detail

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_30032_5ion.nef
Input source #2: Coordindates	5ion.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:6:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:9:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:11:ASP:OD2	2:1:ZN:ZN	unknown	unknown	n/a
1:22:HIS:NE2	2:1:ZN:ZN	unknown	unknown	n/a
1:26:HIS:NE2	2:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
B	1	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20---------
HILYACDSCGDKFLDANSLAQHVRIHTAQ
|||||||||||||||||||||||||||||
HILYACDSCGDKFLDANSLAQHVRIHTAQ

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	29	0	0	100.0

Content subtype: combined_30032_5ion.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	5		No information
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	235		96.6 (chain: A, length: 29)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	519 (1)	noe	93.1 (chain: A, length: 29)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	22 (1)	hbond	55.2 (chain: A, length: 29)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 29, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 148
  - ¹³C chemical shifts: 65
  - ¹⁵N chemical shifts: 22
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	163	148	90.8
¹³C chemical shifts	126	65	51.6
¹⁵N chemical shifts	33	22	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	55	93.2
¹³C chemical shifts	58	15	25.9
¹⁵N chemical shifts	29	21	72.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	104	93	89.4
¹³C chemical shifts	68	50	73.5
¹⁵N chemical shifts	4	1	25.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	15	88.2
¹³C chemical shifts	17	11	64.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	15	13	86.7
¹³C chemical shifts	15	11	73.3

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 29, Sequence coverage: 93.1%
- Total number of restraints: 495
- Weight of restraints: 1.0 (495)
- Potential type of restraints: square-well-parabolic (495)
- Range of distance target values: 2.35, 3.55 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
    None
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 29, Sequence coverage: 55.2%
  - Total number of restraints: 22
  - Weight of restraints: 1.0 (22)
  - Potential type of restraints: square-well-parabolic (22)
  - Range of distance target values: 2.85, 2.85 (Å)
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
      None
  - Distance restraints on contact map

Keywords C2H2 Zinc finger, Miz-1, ZBTB17, transcription factor

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Found 1 Document (1.561 seconds)

BMRB: 30032

Sample

Related entities 1. Zinc finger and BTB domain-containing protein 17, entity 1, : 1 : 5 : 1302 entities Detail

Interaction partners 1. Zinc finger and BTB domain-containing protein 17, entity 1, : 21 interactors Detail

Experiments performed 5 experiments Detail

Instrument

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Sample

Instrument

Sample

Instrument

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Instrument

Sample

NMR combined restraints 4 contents Detail