Dimerization interface of the noncrystalline HIV-1 capsid protein lattice from solid state NMR spectroscopy of tubular assemblies
PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNWMTETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L
Polymer type: polypeptide(L)
Total | 13C | 15N | |
---|---|---|---|
All | 62.9 % (806 of 1281) | 60.4 % (627 of 1038) | 73.7 % (179 of 243) |
Backbone | 80.6 % (716 of 888) | 79.7 % (538 of 675) | 83.6 % (178 of 213) |
Sidechain | 41.6 % (252 of 606) | 43.6 % (251 of 576) | 3.3 % (1 of 30) |
Aromatic | 0.0 % (0 of 78) | 0.0 % (0 of 73) | 0.0 % (0 of 5) |
Methyl | 40.5 % (53 of 131) | 40.5 % (53 of 131) |
1. Capsid protein p24
PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNWMTETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV LSolvent system water, Pressure 1 atm, Temperature 278 K, pH 8.0, Details 15 mM U-15N,13C-Met and U-15N Capsid protein, water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Capsid protein | [U-15N; 13C-Met and U-15N] | 15 mM | |
2 | H2O | natural abundance | 100 % |
Solvent system water, Pressure 1 atm, Temperature 278 K, pH 8.0, Details 15 mM 2-13C-glycerol and U-15N Capsid protein, water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Capsid protein | [2-13C-glycerol; U-15N] | 15 mM | |
4 | H2O | natural abundance | 100 % |
Solvent system water, Pressure 1 atm, Temperature 278 K, pH 8.0, Details 15 mM 2-13C-glycerol, U-15N, unlabeled Tyr and Phe Capsid protein, water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Capsid protein | [2-13C-glycerol, U-15N; unlabeled Tyr and Phe] | 15 mM | |
6 | H2O | natural abundance | 100 % |
Solvent system water, Pressure 1 atm, Temperature 278 K, pH 8.0, Details 7.5 mM Methyl-13C-Met Capsid protein-1, 7.5 mM 15N-indole Capsid protein-2, water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Capsid protein-1 | [Methyl-13C-Met] | 7.5 mM | |
8 | Capsid protein-2 | [15N-indole] | 7.5 mM | |
9 | H2O | natural abundance | 100 % |
Solvent system water, Pressure 1 atm, Temperature 278 K, pH 8.0, Details 15 mM Methyl-13C-Met, 2-13C-indole, U-15N Capsid protein, water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | Capsid protein | [Methyl-13C-Met, 2-13C-indole, U-15N] | 15 mM | |
11 | H2O | natural abundance | 100 % |