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BMRB: 30043

5IQ5

NMR solution structure of Mayaro virus macro domain

Authors

Melekis, E., Tsika, A.C., Bentrop, D., Papageorgiou, N., Coutard, B., Spyroulias, G.A.

Assembly

Macro domain (E.C.2.1.1.-,2.7.7.-,3.1.3.33,3.4.22.-,3.6.1.15,3.6.4.13,2.7.7.48)

Entity

1. Macro domain (E.C.2.1.1.-,2.7.7.-,3.1.3.33,3.4.22.-,3.6.1.15,3.6.4.13,2.7.7.48) (polymer, Thiol state: all free), 166 monomers, 18117.24 Da Detail

MAPAYTVKRA DIATAIEDAV VNAANHRGQV GDGVCRAVAR KWPQAFRNAA TPVGTAKTVK CDETYIIHAV GPNFNNTSEA EGDRDLAAAY RAVAAEINRL SISSVAIPLL STGIFSAGKD RVHQSLSHLL AAMDTTEARV TIYCRDKTWE QKIKTVLQNR HHHHHH

Formula weight

18117.24 Da

Source organism

Mayaro virus (strain Brazil)

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 73.9 %, Completeness (bb): 75.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.9 % (1356 of 1835)	79.9 % (750 of 939)	64.6 % (465 of 720)	80.1 % (141 of 176)
Backbone	75.6 % (745 of 986)	87.8 % (294 of 335)	63.7 % (312 of 490)	86.3 % (139 of 161)
Sidechain	74.7 % (752 of 1007)	75.5 % (456 of 604)	75.8 % (294 of 388)	13.3 % (2 of 15)
Aromatic	10.3 % (13 of 126)	11.1 % (7 of 63)	8.2 % (5 of 61)	50.0 % (1 of 2)
Methyl	88.0 % (183 of 208)	88.5 % (92 of 104)	87.5 % (91 of 104)

1. entity 1

MAPAYTVKRA DIATAIEDAV VNAANHRGQV GDGVCRAVAR KWPQAFRNAA TPVGTAKTVK CDETYIIHAV GPNFNNTSEA EGDRDLAAAY RAVAAEINRL SISSVAIPLL STGIFSAGKD RVHQSLSHLL AAMDTTEARV TIYCRDKTWE QKIKTVLQNR HHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Mayaro virus' macro domain	natural abundance		0.4 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Mayaro virus' macro domain	[U-99% 15N]		0.4 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-15N]-Ala-Leu-Val Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	Mayaro virus' macro domain	[U-15N]-Ala-Leu-Val		0.4 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-14N]-Arg Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	Mayaro virus' macro domain	[U-14N]-Arg		0.4 mM

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-14N]-Asn-Cys Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
6	Mayaro virus' macro domain	[U-14N]-Asn-Cys		0.4 mM

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
7	Mayaro virus' macro domain	[U-100% 13C; U-100% 15N; U-80% 2H]		0.4 mM

Sample #8

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.5 mM Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	Mayaro virus' macro domain	natural abundance		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 5IQ5, Strand ID: A Detail

Release date

2016-12-07

Citation 1

NMR study of non-structural proteins--part I: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Mayaro virus (MAYV)
Melekis, E., Tsika, A.C., Lichiere, J., Chasapis, C.T., Margiolaki, I., Papageorgiou, N., Coutard, B., Bentrop, D., Spyroulias, G.A.
Biomol. NMR Assign. (2015), 9, 191-195, PubMed 25217003 , DOI 10.1007/s12104-014-9572-0 , Abstract

Show more 2 citaions

Citation 2

NMR study of non-structural proteins--part II: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Venezuelan equine encephalitis virus (VEEV)
Papageorgiou, N., Watier, Y., Saunders, L., Coutard, B., Lantez, V., Gould, E.A., Fitch, A.N., Wright, J.P., Canard, B., Margiolaki, I.
Biomol. NMR Assign. (2015), 9, 247-251, PubMed 25291978 , DOI 10.1007/s12104-014-9584-9 , Abstract

Citation 3

Preliminary insights into the non structural protein 3 macro domain of the Mayaro virus by powder diffraction
Melekis, E., Tsika, A.C., Bentrop, D., Papageorgiou, N., Coutard, B.
Zeitschfrift fur Kristallographie (2010), 225, 576-580, DOI 10.1524/zkri.2010.1348

Hide non-primary 2 citaions

Related entities 1. Macro domain (E.C.2.1.1.-,2.7.7.-,3.1.3.33,3.4.22.-,3.6.1.15,3.6.4.13,2.7.7.48), : 1 : 1 : 81 entities Detail

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Mayaro virus' macro domain	[U-99% 15N]		0.4 mM

2 3D CBCA(CO)NH

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

3 2D 1H-15N HSQC

Instrument

Bruker Bruker Avance III High-Definition four-channel - 700 MHz equipped with a cryogenically cooled 5 mm 1H/13C/15N/D Z-gradient probe (TCI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-15N]-Ala-Leu-Val Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	Mayaro virus' macro domain	[U-15N]-Ala-Leu-Val		0.4 mM

4 2D 1H-15N HSQC

Instrument

Bruker Bruker Avance III High-Definition four-channel - 700 MHz equipped with a cryogenically cooled 5 mm 1H/13C/15N/D Z-gradient probe (TCI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-14N]-Arg Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	Mayaro virus' macro domain	[U-14N]-Arg		0.4 mM

5 3D HNCACB

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
7	Mayaro virus' macro domain	[U-100% 13C; U-100% 15N; U-80% 2H]		0.4 mM

6 2D 1H-1H NOESY

Instrument

Bruker Bruker Avance III High-Definition four-channel - 700 MHz equipped with a cryogenically cooled 5 mm 1H/13C/15N/D Z-gradient probe (TCI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Mayaro virus' macro domain	natural abundance		0.4 mM

7 3D HBHA(CO)NH

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

8 3D HNCACB

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

9 3D HNCA

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

10 3D HNCO

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

11 2D 1H-15N HSQC TROSY

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Mayaro virus' macro domain	[U-99% 15N]		0.4 mM

12 3D HN(CO)CA

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

13 3D HCCH-TOCSY

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

14 3D HNHA

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Mayaro virus' macro domain	[U-99% 15N]		0.4 mM

15 3D 1H-15N NOESY

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Mayaro virus' macro domain	[U-99% 15N]		0.4 mM

16 3D 1H-13C NOESY aromatic

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

17 3D 1H-13C NOESY aliphatic

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

18 3D HN(CA)CO

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

19 2D 1H-13C HSQC

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

20 2D 1H-13C HSQC

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

21 3D 1H-13C NOESY

Instrument

Bruker Bruker Avance - 600 MHz equipped with a cryogenically cooled pulsed-field gradient triple-resonance probe (TXI)

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-99% 13C; U-99% 15N] Mayaro virus' macro domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mayaro virus' macro domain	[U-99% 13C; U-99% 15N]		0.4 mM

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_30043_5iq5.nef
Input source #2: Coordindates	5iq5.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

0--------10--------20--------30--------40--------50--------60--------70--------80--------90-------10
MAPAYTVKRADIATAIEDAVVNAANHRGQVGDGVCRAVARKWPQAFRNAATPVGTAKTVKCDETYIIHAVGPNFNNTSEAEGDRDLAAAYRAVAAEINRL
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MAPAYTVKRADIATAIEDAVVNAANHRGQVGDGVCRAVARKWPQAFRNAATPVGTAKTVKCDETYIIHAVGPNFNNTSEAEGDRDLAAAYRAVAAEINRL
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

0-------110-------120-------130-------140-------150-------160-----
SISSVAIPLLSTGIFSAGKDRVHQSLSHLLAAMDTTEARVTIYCRDKTWEQKIKTVLQNRHHHHHH
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SISSVAIPLLSTGIFSAGKDRVHQSLSHLLAAMDTTEARVTIYCRDKTWEQKIKTVLQNRHHHHHH
-------110-------120-------130-------140-------150-------160------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	166	0	0	100.0

Content subtype: combined_30043_5iq5.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1308		91.0 (chain: A, length: 166)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	1976 (1)	noe	88.6 (chain: A, length: 166)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 166, Sequence coverage: 91.0%
- Total number of assignments
  - ¹H chemical shifts: 731
  - ¹³C chemical shifts: 441
  - ¹⁵N chemical shifts: 136
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	939	731	77.8
¹³C chemical shifts	720	441	61.3
¹⁵N chemical shifts	188	136	72.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	335	290	86.6
¹³C chemical shifts	332	152	45.8
¹⁵N chemical shifts	161	136	84.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	604	441	73.0
¹³C chemical shifts	388	289	74.5
¹⁵N chemical shifts	27	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	106	93	87.7
¹³C chemical shifts	106	91	85.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	63	2	3.2
¹³C chemical shifts	61	0	0.0
¹⁵N chemical shifts	2	0	0.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 166, Sequence coverage: 88.6%
- Total number of restraints: 1976
- Weight of restraints: 1.0 (1976)
- Potential type of restraints: upper-bound-parabolic (1976)
- Range of distance target values: 2.81, 8.44 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords ADP ribose binding module, Alphavirus, Mayaro virus, NMR spectroscopy, Viral protein, viral macro domains

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Found 1 Document (0.85 seconds)

BMRB: 30043

Sample #1

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Sample #7

Sample #8

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Macro domain (E.C.2.1.1.-,2.7.7.-,3.1.3.33,3.4.22.-,3.6.1.15,3.6.4.13,2.7.7.48), : 1 : 1 : 81 entities Detail

Experiments performed 21 experiments Detail

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