BMRB: 30045

DIY G-Quadruplexes: Solution structure of d(GGGTTTGGGTTTTGGGAGGG) in sodium

Authors

Dvorkin, S.A., Karsisiotis, A.I., Webba da Silva, M.

Assembly

DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3')

Entity

1. DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3') (polymer, Thiol state: not present), 20 monomers, 6411.040 Da Detail

GGGTTTGGGT TTTGGGAGGG

Formula weight

6411.04 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.0 %, Completeness (bb): 94.3 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	92.0 % (172 of 187)	92.0 % (172 of 187)
Suger, PO4	94.3 % (132 of 140)	94.3 % (132 of 140)
Nucleobase	85.1 % (40 of 47)	85.1 % (40 of 47)
Aromatic	82.5 % (33 of 40)	82.5 % (33 of 40)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3')

GGGTTTGGGT TTTGGGAGGG

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 (±0.2) atm, Temperature 293.15 (±0.2) K, pH 6.8 (±0.2), Details 1 mM DNA, 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA	none		1 (±1.0) mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

Sample #2

Solvent system 100% D2O, Pressure 1 (±0.2) atm, Temperature 293.15 (±0.2) K, pH 6.8 (±0.2), Details 1 mM DNA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	DNA	none		1 (±1.0) mM
5	D2O	natural abundance		100 %

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Release date

2016-03-30

Citation

Encoding canonical DNA quadruplex structure
Dvorkin, S.A., Karsisiotis, A.I., Webba da Silva, M.
Sci. Adv. (2018), 4, eaat3007-eaat3007, PubMed 30182059 , DOI 10.1126/sciadv.aat3007 , Abstract

Experiments performed 2 experiments Detail