BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	84.1 % (918 of 1091)	83.4 % (477 of 572)	85.5 % (365 of 427)	82.6 % (76 of 92)
Backbone	85.6 % (459 of 536)	84.7 % (155 of 183)	86.6 % (233 of 269)	84.5 % (71 of 84)
Sidechain	83.3 % (533 of 640)	82.8 % (322 of 389)	84.8 % (206 of 243)	62.5 % (5 of 8)
Aromatic	67.9 % (38 of 56)	67.9 % (19 of 28)	65.4 % (17 of 26)	100.0 % (2 of 2)
Methyl	86.4 % (102 of 118)	86.4 % (51 of 59)	86.4 % (51 of 59)

1. entity 1

GSLEVLFQGP SPQKPIVRVF LPNKQRTVVP ARCGVTVRDS LKKALMMRGL IPECCAVYRI QDGEKKPIGW DTDISWLTGE ELHVEVLENV PL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-99% 15N] B-RAF RBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	B-RAF RBD	[U-99% 15N]		200 uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-99% 13C; U-99% 15N] B-RAF RBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	B-RAF RBD	[U-99% 13C; U-99% 15N]		200 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 7 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.52 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5J17, Strand ID: A Detail

Release date

2016-09-29

Citation

A Small Molecule RAS-Mimetic Disrupts RAS Association with Effector Proteins to Block Signaling
Athuluri-Divakar, S.K., Vasquez-Del Carpio, R., Dutta, K., Baker, S.J., Cosenza, S.C., Basu, I., Gupta, Y.K., Reddy, M.V., Ueno, L., Hart, J.R., Vogt, P.K., Mulholland, D., Guha, C., Aggarwal, A.K., Reddy, E.P.
Cell (2016), 165, 643-655, PubMed 27104980 , DOI 10.1016/j.cell.2016.03.045 , Abstract

Related entities 1. Serine/threonine-protein kinase B-raf (E.C.2.7.11.1), : 1 : 2 : 7 : 64 entities Detail

Interaction partners 1. Serine/threonine-protein kinase B-raf (E.C.2.7.11.1), : 44 interactors Detail

More details for 44 interactors identified by 15 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-99% 15N] B-RAF RBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	B-RAF RBD	[U-99% 15N]		200 uM

2 2D 1H-13C HSQC