Intermediate state lying on the pathway of release of Tat from HIV-1 TAR.
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 14.6 % (89 of 610) | 23.8 % (84 of 353) | 1.9 % (5 of 257) |
Backbone | 6.8 % (26 of 383) | 12.0 % (24 of 200) | 1.1 % (2 of 183) |
Sidechain | 26.8 % (64 of 239) | 39.2 % (60 of 153) | 4.7 % (4 of 86) |
Aromatic | 0.0 % (0 of 112) | 0.0 % (0 of 64) | 0.0 % (0 of 48) |
Methyl | 57.1 % (8 of 14) | 85.7 % (6 of 7) | 28.6 % (2 of 7) |
1. Cyclic peptide mimetic of HIV-1 Tat
RVRTRKGRRI RIXP2. Apical region (29mer) of the HIV-1 TAR RNA element
GGCAGAUCUG AGCCUGGGAG CUCUCUGCCSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.6, Details 2 uM [U-99% 13C; U-99% 15N] TAR and Tatp, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TAR and Tatp | [U-99% 13C; U-99% 15N] | 2 uM | |
2 | D2O | natural abundance | 100 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 40.1 % (489 of 1220) | 45.6 % (322 of 706) | 32.5 % (167 of 514) |
Backbone | 39.8 % (305 of 766) | 47.2 % (189 of 400) | 31.7 % (116 of 366) |
Sidechain | 38.7 % (185 of 478) | 43.5 % (133 of 306) | 30.2 % (52 of 172) |
Aromatic | 48.2 % (108 of 224) | 46.9 % (60 of 128) | 50.0 % (48 of 96) |
Methyl | 28.6 % (8 of 28) | 42.9 % (6 of 14) | 14.3 % (2 of 14) |
1. Cyclic peptide mimetic of HIV-1 Tat
RVRTRKGRRI RIXP2. Apical region (29mer) of the HIV-1 TAR RNA element
GGCAGAUCUG AGCCUGGGAG CUCUCUGCCSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.6, Details 2 uM [U-99% 13C; U-99% 15N] TAR and Tatp, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TAR and Tatp | [U-99% 13C; U-99% 15N] | 2 uM | |
2 | D2O | natural abundance | 100 % |