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BMRB: 30070

5JN6

The NMR Solution Structure of RPA3313

Authors

Catazaro, J., Lowe, A.J., Powers, R., Structural Genomics Consortium (SGC), S.

Assembly

Uncharacterized protein

Entity

1. Uncharacterized protein (polymer, Thiol state: all free), 91 monomers, 9872.511 Da Detail

MGSSHHHHHH SSGRENLYFQ GMGKAYYDIV GSDNRWGIRH DDDPTGDYSS KEAAFEAACA AASNAIKFGH EVRITVPGND GSETNLGVIT N

Formula weight

9872.511 Da

Source organism

Rhodopseudomonas palustris CGA009

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 75.8 %, Completeness: 67.3 %, Completeness (bb): 75.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.3 % (655 of 973)	67.5 % (336 of 498)	66.1 % (250 of 378)	71.1 % (69 of 97)
Backbone	75.3 % (408 of 542)	73.3 % (140 of 191)	76.0 % (199 of 262)	77.5 % (69 of 89)
Sidechain	60.3 % (308 of 511)	63.8 % (196 of 307)	57.1 % (112 of 196)	0.0 % (0 of 8)
Aromatic	22.6 % (24 of 106)	45.3 % (24 of 53)	0.0 % (0 of 52)	0.0 % (0 of 1)
Methyl	87.1 % (61 of 70)	80.0 % (28 of 35)	94.3 % (33 of 35)

1. Uncharacterized protein

MGSSHHHHHH SSGRENLYFQ GMGKAYYDIV GSDNRWGIRH DDDPTGDYSS KEAAFEAACA AASNAIKFGH EVRITVPGND GSETNLGVIT N

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

LACS Plot; CA

Referencing offset: 2.59 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 2.59 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 2.71 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 5JN6, Strand ID: A Detail

Release date

2016-06-08

Citation

The NMR solution structure and function of RPA3313: a putative ribosomal transport protein from Rhodopseudomonas palustris
Catazaro, J., Lowe, A.J., Cerny, R.L., Powers, R.
Proteins (2017), 85, 93-102, PubMed 27802574 , DOI 10.1002/prot.25201 , Abstract

Related entities 1. Uncharacterized protein, : 1 : 2 entities Detail

Experiments performed 16 experiments Detail

1 3D HNCA

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

2 3D HNCO

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

3 3D HN(CO)CA

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

4 3D HN(CA)CO

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

5 3D CBCA(CO)NH

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

6 3D HBHA(CO)NH

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

7 3D CC(CA)NH

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

8 3D HCC(CO)NH

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

9 3D CC(CO)NH

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

10 3D HCCH-TOCSY

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

11 3D HCCH-COSY

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

12 2D 1H-13C HSQC

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

13 2D 1H-15N HSQC

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

14 3D 1H-15N NOESY

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

15 3D 1H-13C NOESY

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

16 3D HNHA

Instrument

Bruker Avance III - 700 MHz 5mm QCI-P cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RPA3313	none		1 mM
2	MES	natural abundance		18 mM
3	sodium azide	natural abundance		0.1 %
4	sodium chloride	natural abundance		80 mM

nullKeywords SGC, Structural Genomics, Structural Genomics Consortium, UNKNOWN FUNCTION, protein-protein interaction, ribosome