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Found 1 Document (5.561 seconds)

BMRB: 30071

5JOJ

Calcium-loaded EF-hand domain of L-plastin

Authors

Ishida, H., Jensen, K.V., Woodman, G.W., Hyndman, E.M., Vogel, H.J.

Assembly

Plastin-2

Entity

1. Plastin-2 (polymer), 97 monomers, 10953.33 Da Detail

MARGSVSDEE MMELREAFAK VDTDGNGYIS FNELNDLFKA ACLPLPGYRV REITENLMAT GDLDQDGRIS FDEFIKIFHG LKSTDVAKTF RKAINKK

2. CALCIUM ION (non-polymer), 40.078 × 2 Da

Total weight

11033.486 Da

Max. entity weight

10953.33 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 45.0 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.0 % (509 of 1132)	20.8 % (123 of 590)	65.8 % (290 of 441)	95.0 % (96 of 101)
Backbone	83.2 % (481 of 578)	51.3 % (102 of 199)	100.0 % (284 of 284)	100.0 % (95 of 95)
Sidechain	18.3 % (118 of 644)	5.4 % (21 of 391)	38.9 % (96 of 247)	16.7 % (1 of 6)
Aromatic	0.0 % (0 of 90)	0.0 % (0 of 45)	0.0 % (0 of 45)
Methyl	12.2 % (12 of 98)	4.1 % (2 of 49)	20.4 % (10 of 49)

1. Plastin-2

MARGSVSDEE MMELREAFAK VDTDGNGYIS FNELNDLFKA ACLPLPGYRV REITENLMAT GDLDQDGRIS FDEFIKIFHG LKSTDVAKTF RKAINKK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1.0 mM [U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	EF-hand domain of L-plastin	[U-98% 15N]	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]		0.5 mM
8	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.7 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 5JOJ, Strand ID: A Detail

Release date

2016-05-23

Citation

The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling
Ishida, H., Jensen, K.V., Woodman, A.G., Hyndman, M.E., Vogel, H.J.
Sci. Rep. (2017), 7, 40662-40662, PubMed 28145401 , DOI 10.1038/srep40662 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30072 released on 2016-05-23
Title Calcium-free EF-hand domain of L-plastin

Related entities 1. Plastin-2, : 1 : 2 : 1 : 298 entities Detail

Interaction partners 1. Plastin-2, : 10 interactors Detail

More details for 10 interactors identified by 6 citations

Experiments performed 8 experiments Detail

1 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1.0 mM [U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	EF-hand domain of L-plastin	[U-98% 15N]	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

7 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]		0.5 mM
8	D2O	natural abundance		100 %

nullKeywords Calcium-binding, EF-hand, L-plastin

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Found 1 Document (5.561 seconds)

BMRB: 30071

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Plastin-2, : 1 : 2 : 1 : 298 entities Detail

Interaction partners 1. Plastin-2, : 10 interactors Detail

Experiments performed 8 experiments Detail

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