BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	43.0 % (407 of 947)	17.3 % (85 of 492)	65.4 % (242 of 370)	94.1 % (80 of 85)
Backbone	82.2 % (401 of 488)	48.5 % (82 of 169)	100.0 % (239 of 239)	100.0 % (80 of 80)
Sidechain	15.2 % (81 of 534)	0.9 % (3 of 323)	37.9 % (78 of 206)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 80)	0.0 % (0 of 40)	0.0 % (0 of 40)
Methyl	11.0 % (9 of 82)	2.4 % (1 of 41)	19.5 % (8 of 41)

1. Plastin-2

MARGSVSDEE MMELREAFAK VDTDGNGYIS FNELNDLFKA ACLPLPGYRV REITENLMAT GDLDQDGRIS FDEFIKIFHG LK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1 mM [U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	EF-hand domain of L-plastin	[U-98% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]		0.5 mM
8	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 5JOL, Strand ID: A Detail

Release date

2016-05-23

Citation

The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling
Ishida, H., Jensen, K.V., Woodman, A.G., Hyndman, M.E., Vogel, H.J.
Sci. Rep. (2017), 7, 40662-40662, PubMed 28145401 , DOI 10.1038/srep40662 , Abstract

Related entities 1. Plastin-2, : 1 : 3 : 473 entities Detail

Interaction partners 1. Plastin-2, : 10 interactors Detail

More details for 10 interactors identified by 6 citations

Experiments performed 8 experiments Detail

1 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1 mM [U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	EF-hand domain of L-plastin	[U-98% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

7 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-99% 13C; U-98% 15N] EF-hand domain of L-plastin, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	EF-hand domain of L-plastin	[U-99% 13C; U-98% 15N]		0.5 mM
8	D2O	natural abundance		100 %

nullKeywords Calcium-binding, EF-hand, L-plastin

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Found 1 Document (1.772 seconds)

BMRB: 30072

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Plastin-2, : 1 : 3 : 473 entities Detail

Interaction partners 1. Plastin-2, : 10 interactors Detail

Experiments performed 8 experiments Detail

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