Structural Model of a Protein alpha subunit in complex with GDP obtained with SAXS and NMR residual couplings
GASREVKLLL LGAGESGKST IVKQMKIIHE AGYSEEECKQ YKAVVYSNTI QSIIAIIRAM GRLKIDFGDS ARADDARQLF VLAGAAEEGF MTAELAGVIK RLWKDSGVQA CFNRSREYQL NDSAAYYLND LDRIAQPNYI PTQQDVLRTR VKTTGIVETH FTFKDLHFKM FDVGGQRSER KKWIHCFEGV AAIIFCVALS DYDLVLAEDE EMNRMHESMK LFDSICNNKW FTDTSIILFL NKKDLFEEKI KKSPLTICYQ EYAGSNTYEE AAAYIQCQFE DLNKRKDTKE IYTHFTCATD TKNVQFVFDA VTDVIIKNNL KDCGLF
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 26.7 % (629 of 2357) | 19.1 % (382 of 2003) | 69.8 % (247 of 354) |
Backbone | 52.4 % (518 of 989) | 41.1 % (274 of 666) | 75.5 % (244 of 323) |
Sidechain | 8.1 % (111 of 1368) | 8.1 % (108 of 1337) | 9.7 % (3 of 31) |
Aromatic | 5.6 % (10 of 180) | 4.0 % (7 of 177) | 100.0 % (3 of 3) |
Methyl | 12.0 % (22 of 183) | 12.0 % (22 of 183) |
1. Guanine nucleotide-binding protein G(i) subunit alpha-1
GASREVKLLL LGAGESGKST IVKQMKIIHE AGYSEEECKQ YKAVVYSNTI QSIIAIIRAM GRLKIDFGDS ARADDARQLF VLAGAAEEGF MTAELAGVIK RLWKDSGVQA CFNRSREYQL NDSAAYYLND LDRIAQPNYI PTQQDVLRTR VKTTGIVETH FTFKDLHFKM FDVGGQRSER KKWIHCFEGV AAIIFCVALS DYDLVLAEDE EMNRMHESMK LFDSICNNKW FTDTSIILFL NKKDLFEEKI KKSPLTICYQ EYAGSNTYEE AAAYIQCQFE DLNKRKDTKE IYTHFTCATD TKNVQFVFDA VTDVIIKNNL KDCGLFSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details 400 uM [U-95% 2H; U-98% 13C; U-98% 15N] G protein alpha subunit, subtype i,1, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | G protein alpha subunit, subtype i,1 | [U-95% 2H; U-98% 13C; U-98% 15N] | 400 uM | |
2 | H2O | natural abundance | 95 % | |
3 | D2O | natural abundance | 5 % | |
4 | DTT | natural abundance | 5 mM | |
5 | sodium phosphate | natural abundance | 20 mM | |
6 | sodium chloride | natural abundance | 50 mM | |
7 | magnesium chloride | natural abundance | 5 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details 250 uM [U-90% 2H; U-98% 15N] G protein alpha subunit, subtype i,1, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | G protein alpha subunit, subtype i,1 | [U-90% 2H; U-98% 15N] | 250 uM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % | |
11 | DTT | natural abundance | 5 mM | |
12 | sodium phosphate | natural abundance | 20 mM | |
13 | sodium chloride | natural abundance | 50 mM | |
14 | magnesium chloride | natural abundance | 5 mM |