The NMR structure of the m domain tri-helix bundle and C2 of human cardiac Myosin Binding Protein C
GPGSEDVWEI LRQAPPSEYE RIAFQYGVTD LRGMLKRLKG MRRDEKKSTA FQKKLEPAYQ VSKGHKIRLT VELADHDAEV KWLKNGQEIQ MSGSKYIFES IGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.5 % (1545 of 1617) | 96.4 % (819 of 850) | 93.6 % (583 of 623) | 99.3 % (143 of 144) |
Backbone | 97.8 % (796 of 814) | 96.8 % (272 of 281) | 98.0 % (392 of 400) | 99.2 % (132 of 133) |
Sidechain | 94.2 % (875 of 929) | 96.1 % (547 of 569) | 90.8 % (317 of 349) | 100.0 % (11 of 11) |
Aromatic | 64.3 % (72 of 112) | 80.4 % (45 of 56) | 46.3 % (25 of 54) | 100.0 % (2 of 2) |
Methyl | 97.8 % (133 of 136) | 98.5 % (67 of 68) | 97.1 % (66 of 68) |
1. Myosin-binding protein C, cardiac-type
GPGSEDVWEI LRQAPPSEYE RIAFQYGVTD LRGMLKRLKG MRRDEKKSTA FQKKLEPAYQ VSKGHKIRLT VELADHDAEV KWLKNGQEIQ MSGSKYIFES IGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 412 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CaCl2 | natural abundance | 1 mM | |
2 | DSS | natural abundance | 10 mM | |
3 | MES | natural abundance | 10 mM | |
4 | Na azide | natural abundance | 1 mM | |
5 | NaCl | natural abundance | 50 mM | |
6 | TCEP | natural abundance | 2 mM | |
7 | human cMyBP-C (319-451) | [U-99% 13C; U-99% 15N] | 412 uM | |
8 | H2O | natural abundance | 95 % | |
9 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 920 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | CaCl2 | natural abundance | 1 mM | |
11 | DSS | natural abundance | 10 mM | |
12 | MES | natural abundance | 10 mM | |
13 | Na azide | natural abundance | 1 mM | |
14 | NaCl | natural abundance | 50 mM | |
15 | TCEP | natural abundance | 2 mM | |
16 | human cMyBP-C (319-451) | [U-99% 13C; U-99% 15N] | 920 uM | |
17 | H2O | natural abundance | 95 % | |
18 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 550 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 1 mM Na azide, 2 mM TCEP, 10 mM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | CaCl2 | natural abundance | 1 mM | |
20 | DSS | natural abundance | 10 mM | |
21 | MES | natural abundance | 10 mM | |
22 | Na azide | natural abundance | 1 mM | |
23 | NaCl | natural abundance | 50 mM | |
24 | TCEP | natural abundance | 2 mM | |
25 | human cMyBP-C (319-451) | [U-99% 13C; U-99% 15N] | 550 uM | |
26 | H2O | natural abundance | 95 % | |
27 | D2O | natural abundance | 5 % |
Solvent system 99% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 500 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
28 | CaCl2 | natural abundance | 1 mM | |
29 | DSS | natural abundance | 10 mM | |
30 | MES | natural abundance | 10 mM | |
31 | Na azide | natural abundance | 1 mM | |
32 | NaCl | natural abundance | 50 mM | |
33 | TCEP | natural abundance | 2 mM | |
34 | human cMyBP-C (319-451) | [U-99% 13C; U-99% 15N] | 500 uM | |
35 | H2O | natural abundance | 1 % | |
36 | D2O | natural abundance | 99 % |
Solvent system 99% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 800 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
37 | CaCl2 | natural abundance | 1 mM | |
38 | DSS | natural abundance | 10 mM | |
39 | MES | natural abundance | 10 mM | |
40 | Na azide | natural abundance | 1 mM | |
41 | NaCl | natural abundance | 50 mM | |
42 | TCEP | natural abundance | 2 mM | |
43 | human cMyBP-C (319-451) | [U-99% 13C; U-99% 15N] | 800 uM | |
44 | H2O | natural abundance | 1 % | |
45 | D2O | natural abundance | 99 % |