BMRBj - BMREntryMaster

Found 1 Document (1.162 seconds)

BMRB: 30101

6CMY

Structural characterization of melanoregulin (mreg): the protein involved in regulation of cell pigmentation

Authors

Rout, A.K., Bermejo, G., Marie-Paule, S., Hammer, J.A., Tjandra, N.

Assembly

Melanoregulin

Entity

1. Melanoregulin (polymer), 217 monomers, 25312.58 Da Detail

EFSAGLRRWL RSACCCCPCR CLEEPARPEK EPLVSGNNPY SSFGATLERD DEKNLWSMPH DVSHTEADDD RILYNLIVIR NQQTKDSEEW QRLNYDIYTL RQIRREVRNR WRRILEDLGF QREADSLLSV TKLSTMSDSK NTRKAREMLL KLAEETSIFP ASWELSERYL LVVDRLIALD AAEDFFKIAS QMYPKKPGVP CLVDGQRKLH CLPFPSP

Formula weight

25312.58 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.2 %, Completeness: 53.9 %, Completeness (bb): 77.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.9 % (1399 of 2594)	48.8 % (667 of 1367)	57.3 % (575 of 1003)	70.1 % (157 of 224)
Backbone	77.4 % (987 of 1276)	78.2 % (334 of 427)	76.9 % (496 of 645)	77.0 % (157 of 204)
Sidechain	37.7 % (577 of 1529)	35.4 % (333 of 940)	42.9 % (244 of 569)	0.0 % (0 of 20)
Aromatic	9.5 % (18 of 190)	13.7 % (13 of 95)	5.6 % (5 of 90)	0.0 % (0 of 5)
Methyl	52.8 % (115 of 218)	53.2 % (58 of 109)	52.3 % (57 of 109)

1. Melanoregulin

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	melanoregulin	[U-99% 15N]	0.150 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	L-Arginine	natural abundance	5 mM
5	L-Glutamine	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 13C; U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	melanoregulin	[U-99% 13C; U-99% 15N]	0.150 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	L-Arginine	natural abundance	5 mM
10	L-Glutamine	natural abundance	5 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM U-15;U-13C;U-2H melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
11	melanoregulin	U-15;U-13C;U-2H	0.150 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	L-Arginine	natural abundance	5 mM
15	L-Glutamine	natural abundance	5 mM

Sample #4

Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM 15N/13C/2H/ILV-CH3 melanoregulin, 100% D2O

#	Name	Isotope labeling	Concentration
16	melanoregulin	15N/13C/2H/ILV-CH3	0.150 mM
17	D2O	natural abundance	100 %
18	L-Arginine	natural abundance	5 mM
19	L-Glutamine	natural abundance	5 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
20	melanoregulin	[U-99% 15N]	0.150 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %
23	L-Arginine	natural abundance	5 mM
24	L-Glutamine	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	4.78 ppm	external	direct	0.1
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	4.78 ppm	external	direct	0.1
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	4.78 ppm	external	direct	0.1
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	DSS	methyl protons	4.78 ppm	external	direct	0.1
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5KBO, Strand ID: A Detail

Release date

2016-06-07

Citation

The Structure of Melanoregulin Reveals a Role for Cholesterol Recognition in the Protein's Ability to Promote Dynein Function
Rout, A.K., Wu, X., Starich, M.R., Strub, M.P., Hammer, J.A., Tjandra, N.
Structure (2018), 26, 1373-1383, PubMed 30174147 , DOI 10.1016/j.str.2018.07.009 , Abstract

Related entities 1. Melanoregulin, : 2 : 4 entities Detail

Interaction partners 1. Melanoregulin, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM U-15;U-13C;U-2H melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
11	melanoregulin	U-15;U-13C;U-2H	0.150 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	L-Arginine	natural abundance	5 mM
15	L-Glutamine	natural abundance	5 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 13C; U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	melanoregulin	[U-99% 13C; U-99% 15N]	0.150 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	L-Arginine	natural abundance	5 mM
10	L-Glutamine	natural abundance	5 mM

3 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	melanoregulin	U-15;U-13C;U-2H	0.150 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	L-Arginine	natural abundance	5 mM
15	L-Glutamine	natural abundance	5 mM

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	melanoregulin	U-15;U-13C;U-2H	0.150 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	L-Arginine	natural abundance	5 mM
15	L-Glutamine	natural abundance	5 mM

5 3D HN(CO)CACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	melanoregulin	U-15;U-13C;U-2H	0.150 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	L-Arginine	natural abundance	5 mM
15	L-Glutamine	natural abundance	5 mM

6 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	melanoregulin	U-15;U-13C;U-2H	0.150 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	L-Arginine	natural abundance	5 mM
15	L-Glutamine	natural abundance	5 mM

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	melanoregulin	[U-99% 13C; U-99% 15N]	0.150 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	L-Arginine	natural abundance	5 mM
10	L-Glutamine	natural abundance	5 mM

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	melanoregulin	[U-99% 15N]	0.150 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	L-Arginine	natural abundance	5 mM
5	L-Glutamine	natural abundance	5 mM

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	melanoregulin	[U-99% 13C; U-99% 15N]	0.150 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	L-Arginine	natural abundance	5 mM
10	L-Glutamine	natural abundance	5 mM

10 3D HMCMCBCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM 15N/13C/2H/ILV-CH3 melanoregulin, 100% D2O

#	Name	Isotope labeling	Concentration
16	melanoregulin	15N/13C/2H/ILV-CH3	0.150 mM
17	D2O	natural abundance	100 %
18	L-Arginine	natural abundance	5 mM
19	L-Glutamine	natural abundance	5 mM

11 3D ILV-CH3 NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM 15N/13C/2H/ILV-CH3 melanoregulin, 100% D2O

#	Name	Isotope labeling	Concentration
16	melanoregulin	15N/13C/2H/ILV-CH3	0.150 mM
17	D2O	natural abundance	100 %
18	L-Arginine	natural abundance	5 mM
19	L-Glutamine	natural abundance	5 mM

12 2D IPAP

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	melanoregulin	[U-99% 15N]	0.150 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	L-Arginine	natural abundance	5 mM
5	L-Glutamine	natural abundance	5 mM

13 1H_T2

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
20	melanoregulin	[U-99% 15N]	0.150 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %
23	L-Arginine	natural abundance	5 mM
24	L-Glutamine	natural abundance	5 mM

nullKeywords TRANSPORT PROTEIN, alpha helical, cellular pigmentation

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Found 1 Document (1.162 seconds)

BMRB: 30101

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Melanoregulin, : 2 : 4 entities Detail

Interaction partners 1. Melanoregulin, : 4 interactors Detail

Experiments performed 13 experiments Detail

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