BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.2 % (1364 of 1495)	92.5 % (724 of 783)	89.6 % (519 of 579)	91.0 % (121 of 133)
Backbone	92.4 % (684 of 740)	94.0 % (236 of 251)	90.8 % (335 of 369)	94.2 % (113 of 120)
Sidechain	90.6 % (792 of 874)	91.7 % (488 of 532)	90.0 % (296 of 329)	61.5 % (8 of 13)
Aromatic	71.2 % (74 of 104)	80.8 % (42 of 52)	62.0 % (31 of 50)	50.0 % (1 of 2)
Methyl	94.4 % (134 of 142)	95.8 % (68 of 71)	93.0 % (66 of 71)

1. La-related protein 7

MHHHHHHSNA TGPQFVSGVI VKIISTEPLP GRKQVRDTLA AISEVLYVDL LEGDTECHAR FKTPEDAQAV INAYTEINKK HCWKLEILSG DHEQRYWQKI LVDRQAKLNQ PREKKRGTEK LITKA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 298 K, pH 6.1, Details 0.8 mM [U-99% 13C; U-99% 15N] hLARP7 xRRM2, 0.8 mM [U-99% 15N] hLARP7 xRRM2, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

Sample #2

Solvent system 100% D2O, Pressure 760 mmHg, Temperature 298 K, pH 6.1, Details 0.8 mM [U-99% 13C; U-99% 15N] hLARP7 xRRM2, 100% D2O

#	Name	Isotope labeling	Concentration
8	hLARP7 xRRM2	[U-99% 13C; U-99% 15N]	0.8 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaPO4	natural abundance	20 mM
12	NaCl	natural abundance	100 mM
13	TCEP	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5KNW, Strand ID: A Detail

Release date

2016-10-06

Citation

hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA
Eichhorn, C.D., Chug, R., Feigon, J.
Nucleic Acids Res. (2016), 44, 9977-9989, PubMed 27679474 , DOI 10.1093/nar/gkw833 , Abstract

Related entities 1. La-related protein 7, : 2 : 9 entities Detail

Interaction partners 1. La-related protein 7, : 33 interactors Detail

More details for 33 interactors identified by 19 citations

Experiments performed 14 experiments Detail

1 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 298 K, pH 6.1, Details 0.8 mM [U-99% 13C; U-99% 15N] hLARP7 xRRM2, 0.8 mM [U-99% 15N] hLARP7 xRRM2, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

3 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

4 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

5 3d HN(CA)CO

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

6 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

7 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

8 3D HCCH-COSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

10 3D CCCONH

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

11 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

12 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

13 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

14 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	hLARP7 xRRM2-1	[U-99% 13C; U-99% 15N]	0.8 mM
2	hLARP7 xRRM2-2	[U-99% 15N]	0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaPO4	natural abundance	20 mM
6	NaCl	natural abundance	100 mM
7	TCEP	natural abundance	1 mM

NMR combined restraints 2 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts	combined_30126_5knw.nef
Input source #2: Coordindates	5knw.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---440-----450-------460-------470-------480-------490-------500-------510-------520-------530------
MHHHHHHSNATGPQFVSGVIVKIISTEPLPGRKQVRDTLAAISEVLYVDLLEGDTECHARFKTPEDAQAVINAYTEINKKHCWKLEILSGDHEQRYWQKI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MHHHHHHSNATGPQFVSGVIVKIISTEPLPGRKQVRDTLAAISEVLYVDLLEGDTECHARFKTPEDAQAVINAYTEINKKHCWKLEILSGDHEQRYWQKI
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-540-------550-------560-
LVDRQAKLNQPREKKRGTEKLITKA
|||||||||||||||||||||||||
LVDRQAKLNQPREKKRGTEKLITKA
-------110-------120-----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	125	0	0	100.0

Content subtype: combined_30126_5knw.nef

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1370		95.2 (chain: A, length: 125)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 125, Sequence coverage: 95.2%
- Total number of assignments
  - ¹H chemical shifts: 729
  - ¹³C chemical shifts: 520
  - ¹⁵N chemical shifts: 121
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	783	729	93.1
¹³C chemical shifts	579	520	89.8
¹⁵N chemical shifts	140	121	86.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	251	238	94.8
¹³C chemical shifts	250	224	89.6
¹⁵N chemical shifts	120	113	94.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	532	491	92.3
¹³C chemical shifts	329	296	90.0
¹⁵N chemical shifts	20	8	40.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	70	97.2
¹³C chemical shifts	72	65	90.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	52	42	80.8
¹³C chemical shifts	50	31	62.0
¹⁵N chemical shifts	2	1	50.0

Keywords 7SK, RRM, xRRM

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Found 1 Document (1.81 seconds)

BMRB: 30126

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. La-related protein 7, : 2 : 9 entities Detail

Interaction partners 1. La-related protein 7, : 33 interactors Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 2 contents Detail