Solution Structure of a DNA Dodecamer with 5-methylcytosine at the 3rd Position and 9th position
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 57.1 % (56 of 98) | 57.1 % (56 of 98) |
Suger, PO4 | 55.7 % (39 of 70) | 55.7 % (39 of 70) |
Nucleobase | 60.7 % (17 of 28) | 60.7 % (17 of 28) |
Aromatic | 75.0 % (15 of 20) | 75.0 % (15 of 20) |
Methyl | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*(DMC)P*GP*CP*G)-3')
CGCXAATTXG CGSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 50 mM sodium chloride, 10 mM potassium chloride, 0.1 mM EDTA, 1 mM DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*(DMC)P*GP*CP*G)-3'), 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*(DMC)P*GP*CP*G)-3') | natural abundance | 1 mM | |
2 | EDTA | natural abundance | 0.1 mM | |
3 | potassium chloride | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | D2O | natural abundance | 100 % |
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 33.7 % (66 of 196) | 33.7 % (66 of 196) |
Suger, PO4 | 27.9 % (39 of 140) | 27.9 % (39 of 140) |
Nucleobase | 48.2 % (27 of 56) | 48.2 % (27 of 56) |
Aromatic | 52.5 % (21 of 40) | 52.5 % (21 of 40) |
Methyl | 50.0 % (2 of 4) | 50.0 % (2 of 4) |
1. DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*(DMC)P*GP*CP*G)-3')
CGCXAATTXG CGSolvent system 100% D2O, Pressure 1 atm, Temperature 274 K, pH 6.8, Details 50 mM sodium chloride, 10 mM potassium chloride, 0.1 mM EDTA, 1 mM DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*(DMC)P*GP*CP*G)-3'), 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*(DMC)P*GP*CP*G)-3') | natural abundance | 1 mM | |
2 | EDTA | natural abundance | 0.1 mM | |
3 | potassium chloride | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | D2O | natural abundance | 100 % |