BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.3 % (1202 of 1331)	90.9 % (626 of 689)	89.0 % (471 of 529)	92.9 % (105 of 113)
Backbone	94.1 % (634 of 674)	93.9 % (217 of 231)	94.6 % (316 of 334)	92.7 % (101 of 109)
Sidechain	87.5 % (668 of 763)	89.3 % (409 of 458)	84.7 % (255 of 301)	100.0 % (4 of 4)
Aromatic	36.0 % (36 of 100)	46.0 % (23 of 50)	26.0 % (13 of 50)
Methyl	99.3 % (135 of 136)	100.0 % (68 of 68)	98.5 % (67 of 68)

1. Nucleoporin POM152

MSLRVKPSAS LKLHHDLKLC LGDHSSVPVA LKGQGPFTLT YDIIETFSSK RKTFEIKEIK TNEYVIKTPV FTTGGDYILS LVSIKDSTGC VVGLSQPDAK IQVRRDEGHH HHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 235 uM [U-100% 13C; U-100% 15N] POM152, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	POM152	[U-100% 13C; U-100% 15N]	235 uM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	150 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 402 uM [U-100% 13C; U-100% 15N] POM152, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	POM152	[U-100% 13C; U-100% 15N]	402 uM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %
8	NaCl	natural abundance	150 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 300 uM [U-100% 13C; U-100% 15N] POM152, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	POM152	[U-100% 13C; U-100% 15N]	300 uM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %
12	NaCl	natural abundance	150 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1.2 mM [U-100% 13C; U-100% 15N] POM152, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
13	POM152	[U-100% 13C; U-100% 15N]	1.2 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	150 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 324 uM [U-100% 13C; U-100% 15N] POM152, 100% D2O

#	Name	Isotope labeling	Concentration
17	POM152	[U-100% 13C; U-100% 15N]	324 uM
18	D2O	natural abundance	100 %
19	NaCl	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5TVZ, Strand ID: A Detail

Release date

2016-11-21

Citation

Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex
Upla, P., Kim, S.J., Dutta, K., Sampathkumar, P., Cahill, S.M., Chemmama, I.E., Williams, R., Bonanno, J.B., Rice, W.J., Stokes, D.L., Cowburn, D., Almo, S.C., Sali, A., Rout, M.P., Fernandez-Martinez, J.
Structure (2017), 25, 434-445, PubMed 28162953 , DOI 10.1016/j.str.2017.01.006 , Abstract

Entries sharing articles EMDB: 1 entries Detail

EMDB: 8543 released on 2017-01-25
Title 3D negative stain EM structure of Pom152, the major component of the membrane ring of the nuclear pore complex

Related entities 1. Nucleoporin POM152, : 6 entities Detail

Interaction partners 1. Nucleoporin POM152, : 13 interactors Detail

More details for 13 interactors identified by 5 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC