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BMRB: 30207

5U6H

Solution structure of the zinc fingers 1 and 2 of MBNL1

Authors

Phukan, P.D., Park, S., Martinez-Yamout, M.M., Zeeb, M., Dyson, H.J., Wright, P.E.

Assembly

Muscleblind-like protein 1

Entity

1. Muscleblind-like protein 1 (polymer), 92 monomers, 10559.03 Da Detail

MAVSVTPIRD TKWLTLEVCR EFQRGTCSRP DTECKFAHPS KSCQVENGRV IACFDSLKGR CSRENCKYLH PPPHLKTQLE INGRNNLIQQ KN

2. ZINC ION (non-polymer), 112.8027 × 2 Da

Total weight

10784.636 Da

Max. entity weight

10559.03 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 74.6 %, Completeness (bb): 78.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.6 % (812 of 1088)	78.9 % (455 of 577)	66.1 % (273 of 413)	85.7 % (84 of 98)
Backbone	78.1 % (422 of 540)	93.4 % (170 of 182)	62.1 % (169 of 272)	96.5 % (83 of 86)
Sidechain	74.1 % (471 of 636)	72.2 % (285 of 395)	80.8 % (185 of 229)	8.3 % (1 of 12)
Aromatic	37.1 % (23 of 62)	41.9 % (13 of 31)	30.0 % (9 of 30)	100.0 % (1 of 1)
Methyl	89.0 % (73 of 82)	90.2 % (37 of 41)	87.8 % (36 of 41)

1. Muscleblind-like protein 1

MAVSVTPIRD TKWLTLEVCR EFQRGTCSRP DTECKFAHPS KSCQVENGRV IACFDSLKGR CSRENCKYLH PPPHLKTQLE INGRNNLIQQ KN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.4 mM [U-98% 13C; U-98% 15N] Zinc fingers 1 and 2 of MBNL1, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Zinc fingers 1 and 2 of MBNL1	[U-98% 13C; U-98% 15N]	0.4 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	4.7 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	4.7 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	4.7 ppm	external	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	4.7 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	4.7 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	4.7 ppm	external	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	4.7 ppm	external	indirect	0.2514495
¹H	DSS	methyl protons	4.7 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	4.7 ppm	external	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5U6H, Strand ID: A Detail

Release date

2017-02-01

Citation

Structural basis for interaction of the tandem zinc finger domains of human muscleblind with cognate RNA from human cardiac troponin T
Park, S., Phukan, P.D., Zeeb, M., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.
Biochemistry (2017), 56, 4154-4168, PubMed 28718627 , DOI 10.1021/acs.biochem.7b00484 , Abstract

Related entities 1. Muscleblind-like protein 1, : 1 : 7 : 1 : 42 entities Detail

Interaction partners 1. Muscleblind-like protein 1, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 3D HNCA