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BMRB: 30208

5U6L

Solution structure of the zinc fingers 3 and 4 of MBNL1

Authors

Phukan, P.D., Park, S., Martinez-Yamout, M.M., Zeeb, M., Dyson, H.J., Wright, P.E.

Assembly

Muscleblind-like protein 1

Entity

1. Muscleblind-like protein 1 (polymer), 83 monomers, 9654.862 Da Detail

AQKLMRTDRL EVCREYQRGN CNRGENDCRF AHPADSTMID TNDNTVTVCM DYIKGRCSRE KCKYFHPPAH LQAKIKAAQY QVN

2. ZINC ION (non-polymer), 112.8027 × 2 Da

Total weight

9880.468 Da

Max. entity weight

9654.862 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 72.2 %, Completeness (bb): 77.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.2 % (697 of 965)	77.6 % (396 of 510)	62.6 % (228 of 364)	80.2 % (73 of 91)
Backbone	77.8 % (383 of 492)	91.6 % (152 of 166)	64.6 % (159 of 246)	90.0 % (72 of 80)
Sidechain	69.8 % (386 of 553)	70.9 % (244 of 344)	71.2 % (141 of 198)	9.1 % (1 of 11)
Aromatic	31.3 % (20 of 64)	56.3 % (18 of 32)	6.3 % (2 of 32)
Methyl	93.8 % (60 of 64)	93.8 % (30 of 32)	93.8 % (30 of 32)

1. Muscleblind-like protein 1

AQKLMRTDRL EVCREYQRGN CNRGENDCRF AHPADSTMID TNDNTVTVCM DYIKGRCSRE KCKYFHPPAH LQAKIKAAQY QVN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.4 mM [U-98% 13C; U-98% 15N] Zinc fingers 3 and 4 of MBNL1, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Zinc fingers 3 and 4 of MBNL1	[U-98% 13C; U-98% 15N]	0.4 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5U6L, Strand ID: A Detail

Release date

2017-02-01

Citation

Structural basis for interaction of the tandem zinc finger domains of human muscleblind with cognate RNA from human cardiac troponin T
Park, S., Phukan, P.D., Zeeb, M., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.
Biochemistry (2017), 56, 4154-4168, PubMed 28718627 , DOI 10.1021/acs.biochem.7b00484 , Abstract

Related entities 1. Muscleblind-like protein 1, : 1 : 5 : 3 : 84 entities Detail

Interaction partners 1. Muscleblind-like protein 1, : 21 interactors Detail

More details for 21 interactors identified by 7 citations

Experiments performed 11 experiments Detail

1 3D HNCA