NMR SOLUTION STRUCTURE OF THE ALPHA-CONOTOXIN GID MUTANT V13Y
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 72.4 % (126 of 174) | 92.2 % (94 of 102) | 44.4 % (32 of 72) |
Backbone | 70.8 % (63 of 89) | 97.1 % (34 of 35) | 53.7 % (29 of 54) |
Sidechain | 74.8 % (77 of 103) | 89.6 % (60 of 67) | 47.2 % (17 of 36) |
Aromatic | 33.3 % (4 of 12) | 66.7 % (4 of 6) | 0.0 % (0 of 6) |
Methyl | 60.0 % (6 of 10) | 100.0 % (5 of 5) | 20.0 % (1 of 5) |
1. Alpha-conotoxin GID
IRDECCSNPA CRYNNXHVCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 280 K, pH 3, Details 1.5 mM design 8, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | design 8 | none | 1.5 (±0.3) mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 280 K, pH 3, Details 1.5 mM design 8, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | design 8 | none | 1.5 (±0.3) mM | |
5 | D2O | natural abundance | 100 % |