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BMRB: 30234


30234
5UJL
Representative 1-conformer ensembles of K27-linked Ub2 from RDC data
Authors
Castaneda, C.A., Fushman, D.
Assembly
Ubiquitin
Entity
1. Ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 × 2 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG


Total weight
17129.463 Da
Max. entity weight
8564.731 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
na
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 92.1 %, Completeness: 30.8 %, Completeness (bb): 64.5 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All30.8 % (172 of 559)21.3 % (102 of 478)86.4 % (70 of 81)
Backbone64.5 % (147 of 228)49.7 % (77 of 155)95.9 % (70 of 73)
Sidechain 7.6 % (25 of 331) 7.7 % (25 of 323) 0.0 % (0 of 8)
Aromatic 0.0 % (0 of 16) 0.0 % (0 of 16)
Methyl12.2 % (6 of 49)12.2 % (6 of 49)

1. entity 1

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Show sample condition
Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8, Details 150 uM [U-99% 15N] K27-diubiquitin, Distal Ub is 15N-labelled Proximal Ub is not enriched, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1K27-diubiquitin, Distal Ub is 15N-labelled Proximal Ub is not enriched[U-99% 15N]150 uM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8, Details 150 uM [U-99% 15N] K27-diubiquitin, Distal Ub is not enriched Proximal Ub is 15N-labelled, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
2K27-diubiquitin, Distal Ub is not enriched Proximal Ub is 15N-labelled[U-99% 15N]150 uM

Chem. Shift Complete2
Sequence coverage: 92.1 %, Completeness: 29.2 %, Completeness (bb): 64.0 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All29.2 % (327 of 1118)19.5 % (186 of 956)87.0 % (141 of 162)
Backbone64.0 % (292 of 456)49.0 % (152 of 310)95.9 % (140 of 146)
Sidechain 5.4 % (36 of 662) 5.4 % (35 of 646) 6.3 % (1 of 16)
Aromatic 0.0 % (0 of 32) 0.0 % (0 of 32)
Methyl 7.1 % (7 of 98) 7.1 % (7 of 98)

1. entity 1

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Show sample condition
Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8, Details 150 uM [U-99% 15N] K27-diubiquitin, Distal Ub is 15N-labelled Proximal Ub is not enriched, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1K27-diubiquitin, Distal Ub is 15N-labelled Proximal Ub is not enriched[U-99% 15N]150 uM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8, Details 150 uM [U-99% 15N] K27-diubiquitin, Distal Ub is not enriched Proximal Ub is 15N-labelled, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
2K27-diubiquitin, Distal Ub is not enriched Proximal Ub is 15N-labelled[U-99% 15N]150 uM

Protein Blocks Logo
Calculated from 2 models in PDB: 5UJL, Strand ID: A, B Detail

Release date
2017-10-16
Citation
Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins
Castaneda, C.A., Dixon, E.K., Walker, O., Chaturvedi, A., Nakasone, M.A., Curtis, J.E., Reed, M.R., Krueger, S., Cropp, T.A., Fushman, D.
Structure (2016), 24, 423-436, PubMed 26876099 , DOI 10.1016/j.str.2016.01.007 ,
Related entities 1. Ubiquitin, : 1 : 292 : 11 : 72 : 321 entities Detail
More details for 697 related entities
Interaction partners 1. Ubiquitin, : 27 interactors Detail
More details for 27 interactors identified by 16 citations
Experiments performed 6 experiments Detail
nullKeywords K27-linkage, SIGNALING PROTEIN, diubiquitin, post-translational modification