BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.2 % (1249 of 1385)	87.7 % (626 of 714)	91.4 % (492 of 538)	98.5 % (131 of 133)
Backbone	97.7 % (721 of 738)	97.3 % (252 of 259)	97.8 % (350 of 358)	98.3 % (119 of 121)
Sidechain	84.0 % (636 of 757)	82.2 % (374 of 455)	86.2 % (250 of 290)	100.0 % (12 of 12)
Aromatic	42.0 % (42 of 100)	42.0 % (21 of 50)	37.0 % (17 of 46)	100.0 % (4 of 4)
Methyl	96.5 % (137 of 142)	98.6 % (70 of 71)	94.4 % (67 of 71)

1. entity 1

SEFGSMAGNC GARGALSAHT LLFDLPPALL GELCAVLDSC DGALGWRGLA ERLSSSWLDV DHIEKEVDQG KSGTRELLWS WAQKNKTIGD LLQVLQEMGH RRAIHLITNY GAVLSPSEKS YQEG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.67, Details 800 uM [U-99% 13C; U-99% 15N] IRAK-M Death Domain_R56D_Y61E, 20 mM sodium chloride, 5 mM TCEP, 10 mM TRIS, 5 mM sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514496
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.101329

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514496
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.101329

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514496
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.101329

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514496
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.101329

Referencing offset: -0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5UKE, Strand ID: A Detail

Release date

2017-02-12

Citation

The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation
Nechama, M., Kwon, J., Wei, S., Kyi, A.T., Welner, R.S., Ben-Dov, I.Z., Arredouani, M.S., Asara, J.M., Chen, C.H., Tsai, C.Y., Nelson, K.F., Kobayashi, K.S., Israel, E., Zhou, X.Z., Nicholson, L.K., Lu, K.P.
Nat. Commun. (2018), 9, 1603-1603, PubMed 29686383 , DOI 10.1038/s41467-018-03886-6 , Abstract

Related entities 1. Interleukin-1 receptor-associated kinase 3 (E.C.2.7.11.1), : 1 : 2 : 13 entities Detail

Interaction partners 1. Interleukin-1 receptor-associated kinase 3 (E.C.2.7.11.1), : 14 interactors Detail

More details for 14 interactors identified by 4 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.67, Details 800 uM [U-99% 13C; U-99% 15N] IRAK-M Death Domain_R56D_Y61E, 20 mM sodium chloride, 5 mM TCEP, 10 mM TRIS, 5 mM sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

4 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

5 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

6 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

7 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

8 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

9 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

10 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

11 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

12 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

13 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

14 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	IRAK-M Death Domain_R56D_Y61E	[U-99% 13C; U-99% 15N]	800 uM
2	TCEP	natural abundance	5 mM
3	TRIS	natural abundance	10 mM
4	sodium azide	natural abundance	5 mM
5	sodium chloride	natural abundance	20 mM

nullKeywords Asthma, Death Domain, IRAK-M, Innate immunity, NMR

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Found 1 Document (0.913 seconds)

BMRB: 30237

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Interleukin-1 receptor-associated kinase 3 (E.C.2.7.11.1), : 1 : 2 : 13 entities Detail

Interaction partners 1. Interleukin-1 receptor-associated kinase 3 (E.C.2.7.11.1), : 14 interactors Detail

Experiments performed 14 experiments Detail

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