BMRB: 30261

HnRNP A1 Alters the Conformation of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation

Authors

Tolbert, M., Morgan, C.E., Tolbert, B.S.

Assembly

RNA (41-MER)

Entity

1. RNA (41-MER) (polymer, Thiol state: not present), 41 monomers, 13203.83 Da Detail

GGAUCAAUAG CAGGUGUGGC ACACCAGUCA UACCUUGAUC C

Formula weight

13203.83 Da

Source organism

Enterovirus A71

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 36.5 %, Completeness (bb): 32.1 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	36.5 % (131 of 359)	36.5 % (131 of 359)
Suger, PO4	32.1 % (79 of 246)	32.1 % (79 of 246)
Nucleobase	46.0 % (52 of 113)	46.0 % (52 of 113)
Aromatic	57.1 % (52 of 91)	57.1 % (52 of 91)

1. entity 1

GGAUCAAUAG CAGGUGUGGC ACACCAGUCA UACCUUGAUC C

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 null, Temperature 303 K, pH 6.5, Details 180.0 uM Selective Deuteration; EQUIMOLAR MIX:ATP, GTP, CTP, UTP rNTP, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	rNTP	Selective Deuteration; EQUIMOLAR MIX:ATP, GTP, CTP, UTP		180.0 (±0.2) uM

Sample #2

Solvent system 100% D2O, Pressure 1 null, Temperature 303 K, pH 6.5, Details 300.0 uM Fully protonated rNTP, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	rNTP	Fully protonated		300.0 (±0.2) uM

Sample #3

Solvent system 100% D2O, Pressure 1 null, Temperature 303 K, pH 6.5, Details 100.0 uM Fully protonated rNTP, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	rNTP	Fully protonated		100.0 uM

Sample #4

Solvent system 100% D2O, Pressure 1 null, Temperature 303 K, pH 6.5, Details 300.0 uM [U-13C]-Gua rNTP, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	rNTP	[U-13C]-Gua		300.0 uM

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Release date

2017-07-05

Citation

HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation
Tolbert, M., Morgan, C.E., Pollum, M., Crespo-Hernandez, C.E., Li, M.L., Brewer, G., Tolbert, B.S.
J. Mol. Biol. (2017), 429, 2841-2858, PubMed 28625847 , DOI 10.1016/j.jmb.2017.06.007 , Abstract

Experiments performed 4 experiments Detail