BMRB: 30262

HnRNP A1 Alters the Conformation of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation

Authors

Tolbert, M., Morgan, C.E., Tolbert, B.S.

Assembly

RNA (41-MER)

Entity

1. RNA (41-MER) (polymer, Thiol state: not present), 41 monomers, 13138.79 Da Detail

GGAUCAACCC CAGGUGUGGC ACACCAGUCA UACCUUGAUC C

Formula weight

13138.79 Da

Source organism

Enterovirus A71

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 30.2 %, Completeness (bb): 16.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	30.2 % (110 of 364)	30.2 % (110 of 364)
Suger, PO4	16.7 % (41 of 246)	16.7 % (41 of 246)
Nucleobase	58.5 % (69 of 118)	58.5 % (69 of 118)
Aromatic	76.7 % (69 of 90)	76.7 % (69 of 90)

1. entity 1

GGAUCAACCC CAGGUGUGGC ACACCAGUCA UACCUUGAUC C

Show sample condition

Sample #1

Solvent system 100% D20, Pressure 1 null, Temperature 303 K, pH 6.5, Details 220 uM Fully protonated rNTP, 100% D20

#	Name	Isotope labeling	Type	Concentration
1	rNTP	Fully protonated		220 (±0.2) uM

Sample #2

Solvent system 100% D2O, Pressure 1 null, Temperature 303 K, pH 6.5, Details 300 uM Fully protonated rNTP, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	rNTP	Fully protonated		300 (±0.2) uM

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Release date

2017-07-05

Citation

HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation
Tolbert, M., Morgan, C.E., Pollum, M., Crespo-Hernandez, C.E., Li, M.L., Brewer, G., Tolbert, B.S.
J. Mol. Biol. (2017), 429, 2841-2858, PubMed 28625847 , DOI 10.1016/j.jmb.2017.06.007 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 2 experiments Detail