BMRB: 30268

Structure of a GA Rich 8x8 Nucleotide RNA Internal Loop

Authors

Kauffmann, A.D., Kennedy, S.D., Turner, D.H.

Assembly

RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')

Entity

1. RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3') (polymer, Thiol state: not present), 15 monomers, 4940.989 × 2 Da Detail

CCAGAAACGG AUGGA

Total weight

9881.978 Da

Max. entity weight

4940.989 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 71.1 %, Completeness (bb): 73.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H	31P
All	71.1 % (101 of 142)	69.3 % (88 of 127)	86.7 % (13 of 15)
Suger, PO4	73.3 % (77 of 105)	71.1 % (64 of 90)	86.7 % (13 of 15)
Nucleobase	64.9 % (24 of 37)	64.9 % (24 of 37)
Aromatic	77.4 % (24 of 31)	77.4 % (24 of 31)

1. RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')

CCAGAAACGG AUGGA

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 6.2, Details 3.1 mM None RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3'), 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')	None		3.1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 6.2, Details 3.1 mM None RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3'), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')	None		3.1 mM

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Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 56.0 %, Completeness (bb): 48.1 % Detail

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• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polyribonucleotide

	Total	1H	31P
All	56.0 % (159 of 284)	57.5 % (146 of 254)	43.3 % (13 of 30)
Suger, PO4	48.1 % (101 of 210)	48.9 % (88 of 180)	43.3 % (13 of 30)
Nucleobase	78.4 % (58 of 74)	78.4 % (58 of 74)
Aromatic	87.1 % (54 of 62)	87.1 % (54 of 62)

1. RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')

CCAGAAACGG AUGGA

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.2, Details 3.1 mM None RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3'), 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')	None		3.1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.2, Details 3.1 mM None RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3'), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3')	None		3.1 mM

Hide sample codition

Release date

2017-06-19

Citation

Nuclear Magnetic Resonance Structure of an 8 × 8 Nucleotide RNA Internal Loop Flanked on Each Side by Three Watson-Crick Pairs and Comparison to Three-Dimensional Predictions
Kauffmann, A.D., Kennedy, S.D., Zhao, J., Turner, D.H.
Biochemistry (2017), 56, 3733-3744, PubMed 28700212 , DOI 10.1021/acs.biochem.7b00201 , Abstract

Related entities 1. RNA (5'-R(*CP*CP*AP*GP*AP*AP*AP*CP*GP*GP*AP*UP*GP*GP*A)-3'), : 1 : 1 entities Detail

Experiments performed 5 experiments Detail