BMRB: 30273

SSNMR Structure of the Human RIP1/RIP3 Necrosome

Authors

Mompean, M., Li, W., Li, J., Laage, S., Siemer, A.B., Wu, H., McDermott, A.E.

Assembly

Human RIP1/RIP3 complex

Entity

1. Human RIP1/RIP3 complex, entity 1 (polymer), 15 monomers, 1577.757 × 4 Da Detail

PLVNIYNCSG VQVGD

2. Human RIP1/RIP3 complex, entity 2 (polymer, Thiol state: not present), 18 monomers, 2051.277 × 4 Da Detail

TIYNSTGIQI GAYNYMEI

Total weight

14516.137 Da

Max. entity weight

2051.277 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamicsmolecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 79.7 %, Completeness (bb): 89.0 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	79.7 % (145 of 182)	81.3 % (117 of 144)	73.7 % (28 of 38)
Backbone	89.0 % (113 of 127)	90.5 % (86 of 95)	84.4 % (27 of 32)
Sidechain	66.7 % (56 of 84)	70.5 % (55 of 78)	16.7 % (1 of 6)
Aromatic	0.0 % (0 of 16)	0.0 % (0 of 16)
Methyl	81.0 % (17 of 21)	81.0 % (17 of 21)

1. entity 1

PLVNIYNCSG VQVGD

2. entity 2

TIYNSTGIQI GAYNYMEI

Show sample condition

Sample #1

Solvent system 100% H2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 15 mg/mL [U-100% 13C; U-100% 15N] RIP1/RIP3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		15 mg/mL
2	entity_2	[U-100% 13C; U-100% 15N]		15 mg/mL

Sample #2

Solvent system 100% H2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 15 mg/mL [U-100% 15N; 2-13C-GLYCEROL] RIP1/RIP3

#	Name	Isotope labeling	Type	Concentration
3	entity_1	[U-100% 13C; U-100% 15N]		15 mg/mL
4	entity_2	[U-100% 13C; U-100% 15N]		15 mg/mL

Sample #3

Solvent system 100% H2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 15 mg/mL [U-100% 15N; 1,3-13C-GLYCEROL] RIP1/RIP3

#	Name	Isotope labeling	Type	Concentration
5	entity_1	[U-100% 13C; U-100% 15N]		15 mg/mL
6	entity_2	[U-100% 13C; U-100% 15N]		15 mg/mL

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 5V7Z, Strand ID: A, B, C, D, E, F, G, H Detail

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Release date

2018-01-11

Citation

The Structure of the Necrosome RIPK1-RIPK3 Core, a Human Hetero-Amyloid Signaling Complex
Mompean, M., Li, W., Li, J., Laage, S., Siemer, A.B., Bozkurt, G., Wu, H., McDermott, A.E.
Cell (2018), 173, 1244-1253, PubMed 29681455 , DOI 10.1016/j.cell.2018.03.032 , Abstract

Related entities 1. Human RIP1/RIP3 complex, entity 1, : 1 : 2 entities Detail

Related entities 2. Human RIP1/RIP3 complex, entity 2, : 1 : 2 : 6 entities Detail

Interaction partners 1. Human RIP1/RIP3 complex, entity 1, : 93 : 1 interactors Detail

Interaction partners 2. Human RIP1/RIP3 complex, entity 2, : 61 : 1 interactors Detail

Experiments performed 28 experiments Detail