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BMRB: 30283


30283
5VH8
Structure and dynamics of RNA repeat expansions that cause Huntington's Disease and myotonic dystrophy type 1
Authors
Chen, J.L., VanEtten, D.M., Fountain, M.A., Yildirim, I., Disney, M.D.
Assembly
RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3')
Entity
1. RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3') (polymer, Thiol state: not present), 15 monomers, 4823.821 × 2 Da Detail

GACCUGCUGC UGGUC


Total weight
9647.642 Da
Max. entity weight
4823.821 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 39.6 %, Completeness (bb): 34.4 % Detail
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Polymer type: polyribonucleotide

Total1H
All39.6 % (53 of 134)39.6 % (53 of 134)
Suger, PO434.4 % (31 of 90)34.4 % (31 of 90)
Nucleobase50.0 % (22 of 44)50.0 % (22 of 44)
Aromatic64.7 % (22 of 34)64.7 % (22 of 34)

1. entity 1

GACCUGCUGC UGGUC

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 100% D2O.


#NameIsotope labelingTypeConcentration
1EDTAnatural abundance0.25 mM
2RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3')NA Adenosine, Cytidine,Guanosine, Uridine0.5 mM
3potassium phosphatenatural abundance5.0 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 uM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), 5.0 mM potassium phosphate, 0.25 nM EDTA, 90% H2O/10% D2O.


#NameIsotope labelingTypeConcentration
4EDTAnatural abundance0.25 nM
5RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3')NA Adenosine, Cytidine,Guanosine, Uridine0.5 uM
6potassium phosphatenatural abundance5.0 mM

Chem. Shift Complete2
Sequence coverage: 100.0 %, Completeness: 35.1 %, Completeness (bb): 20.0 % Detail
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Polymer type: polyribonucleotide

Total1H
All35.1 % (94 of 268)35.1 % (94 of 268)
Suger, PO420.0 % (36 of 180)20.0 % (36 of 180)
Nucleobase65.9 % (58 of 88)65.9 % (58 of 88)
Aromatic70.6 % (48 of 68)70.6 % (48 of 68)

1. entity 1

GACCUGCUGC UGGUC

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 276 K, pH 6, Details 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 100% D2O.


#NameIsotope labelingTypeConcentration
1EDTAnatural abundance0.25 mM
2RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3')NA Adenosine, Cytidine,Guanosine, Uridine0.5 mM
3potassium phosphatenatural abundance5.0 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 276 K, pH 6, Details 0.5 uM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), 5.0 mM potassium phosphate, 0.25 nM EDTA, 90% H2O/10% D2O.


#NameIsotope labelingTypeConcentration
4EDTAnatural abundance0.25 nM
5RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3')NA Adenosine, Cytidine,Guanosine, Uridine0.5 uM
6potassium phosphatenatural abundance5.0 mM

Release date
2017-04-26
Citation
Structure and dynamics of RNA repeat expansions that cause Huntington's Disease and myotonic dystrophy type 1
Chen, J.L., VanEtten, D.M., Fountain, M.A., Yildirim, I., Disney, M.D.
Biochemistry (2017), 56, 3463-3474, PubMed 28617590 , DOI 10.1021/acs.biochem.7b00252 ,
Related entities 1. RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), : 1 : 1 : 4 entities Detail
More details for 6 related entities
Experiments performed 2 experiments Detail
nullKeywords CUG repeat, UU pair, myotonic dystrophy, repeat expansion, triplet repeat