BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	76.1 % (606 of 796)	68.1 % (286 of 420)	82.1 % (257 of 313)	100.0 % (63 of 63)
Backbone	95.7 % (354 of 370)	95.1 % (117 of 123)	94.7 % (178 of 188)	100.0 % (59 of 59)
Sidechain	63.9 % (312 of 488)	56.9 % (169 of 297)	74.3 % (139 of 187)	100.0 % (4 of 4)
Aromatic	75.6 % (68 of 90)	75.6 % (34 of 45)	75.0 % (33 of 44)	100.0 % (1 of 1)
Methyl	79.6 % (43 of 54)	81.5 % (22 of 27)	77.8 % (21 of 27)

1. entity 1

LPAHPYDRLK TTSTDPVSDI DVTRREAYLS SEEFKEKFGM TKEAFYKLPK WKQNKFKMAV QLF

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1.0 mM atVHP, 20.0 mM PIPES, 50 mM sodium chloride, 0.01 % sodium azide, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	PIPES	none	20.0 mM
2	atVHP	none	1.0 mM
3	sodium azide	none	0.01 %
4	sodium chloride	none	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1.0 mM atVHP, 20.0 mM PIPES, 50 mM sodium chloride, 0.01 % sodium azide, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
5	PIPES	none	20.0 mM
6	atVHP	none	1.0 mM
7	sodium azide	none	0.01 %
8	sodium chloride	none	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	43.0 ppm	internal	indirect	1.0
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	117.5 ppm	internal	indirect	1.0

Referencing offset: 0.26 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	43.0 ppm	internal	indirect	1.0
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	117.5 ppm	internal	indirect	1.0

Referencing offset: 0.26 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	43.0 ppm	internal	indirect	1.0
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	117.5 ppm	internal	indirect	1.0

Referencing offset: 0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	43.0 ppm	internal	indirect	1.0
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	nitrogen	117.5 ppm	internal	indirect	1.0

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5VNT, Strand ID: A Detail

Release date

2017-05-03

Citation

Plant Villin Headpiece Domain Demonstrates a Novel Surface Charge Pattern and High Affinity for F-Actin
Miears, H.L., Gruber, D.R., Horvath, N.M., Antos, J.M., Young, J., Sigurjonsson, J.P., Klem, M.L., Rosenkranz, E.A., Okon, M., McKnight, C.J., Vugmeyster, L., Smirnov, S.L.
Biochemistry (2018), 57, 1690-1701, PubMed 29444403 , DOI 10.1021/acs.biochem.7b00856 , Abstract

Related entities 1. Villin-4, : 1 : 2 : 107 entities Detail

Experiments performed 13 experiments Detail

1 15N-HSQC

Instrument

Bruker AvanceIII - 500 MHz TCI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1.0 mM atVHP, 20.0 mM PIPES, 50 mM sodium chloride, 0.01 % sodium azide, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	PIPES	none	20.0 mM
2	atVHP	none	1.0 mM
3	sodium azide	none	0.01 %
4	sodium chloride	none	50 mM

2 13C-HSQC

Instrument

Bruker AvanceIII - 600 MHz TCI probe