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1H, 13C, 15N chemical shift assignments of the HIV-1 gp41 cytoplasmic tail, residues 752-856
Authors
Saad, J.S., Murphy, R.E., Samal, A., Vlach, J.
Assembly
Cytoplasmic Tail of HIV-1 gp41 protein
Entity
1. Cytoplasmic Tail of HIV-1 gp41 protein (polymer, Thiol state: all free), 105 monomers, 12381.38 Da Detail

SLALIWDDLR SLCLFSYHRL RDLLLIVTRI VELLGRRGWE ALKYWWNLLQ YWSQELKNSA VNLLNATAIA VAEGTDRVIE VLQAAYRAIR HIPRRIRQGL ERILL


Formula weight
12381.38 Da
Source organism
Human immunodeficiency virus 1
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.1 %, Completeness: 87.2 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All87.2 % (1134 of 1300)83.4 % (561 of 673)91.2 % (465 of 510)92.3 % (108 of 117)
Backbone92.0 % (578 of 628)90.6 % (193 of 213)93.2 % (290 of 311)91.3 % (95 of 104)
Sidechain84.5 % (653 of 773)80.0 % (368 of 460)90.7 % (272 of 300)100.0 % (13 of 13)
Aromatic92.7 % (102 of 110)100.0 % (55 of 55)84.0 % (42 of 50)100.0 % (5 of 5)
Methyl85.3 % (145 of 170)78.8 % (67 of 85)91.8 % (78 of 85)

1. entity 1

SLALIWDDLR SLCLFSYHRL RDLLLIVTRI VELLGRRGWE ALKYWWNLLQ YWSQELKNSA VNLLNATAIA VAEGTDRVIE VLQAAYRAIR HIPRRIRQGL ERILL

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 324 K, pH 6.0, Details 500 uM gp4CTc, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM TCEP, 25 mM DPC, 95% H2O/5% D2O.


#NameIsotope labelingTypeConcentration
1DPCnatural abundance25 mM
2TCEPnatural abundance1 mM
3gp4CTcnatural abundance500 uM
4sodium chloridenatural abundance50 mM
5sodium phosphatenatural abundance50 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 324 K, pH 6.0, Details 500 uM [U-99% 15N] gp4CTc, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM TCEP, 25 mM DPC, 95% H2O/5% D2O.


#NameIsotope labelingTypeConcentration
6DPCnatural abundance25 mM
7TCEPnatural abundance1 mM
8gp4CTc[U-99% 15N]500 uM
9sodium chloridenatural abundance50 mM
10sodium phosphatenatural abundance50 mM
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 324 K, pH 6.0, Details 500 uM [U-99% 13C; U-99% 15N] gp4CTc, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM TCEP, 25 mM DPC, 95% H2O/5% D2O.


#NameIsotope labelingTypeConcentration
11DPCnatural abundance25 mM
12TCEPnatural abundance1 mM
13gp4CTc[U-99% 13C; U-99% 15N]500 uM
14sodium chloridenatural abundance50 mM
15sodium phosphatenatural abundance50 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 5VWL, Strand ID: A Detail


Release date
2017-05-24
Citation
Solution Structure and Membrane Interaction of the Cytoplasmic Tail of HIV-1 gp41 Protein
Murphy, R.E., Samal, A.B., Vlach, J., Saad, J.S.
Structure (2017), 25, 1708-1718, PubMed 29056482 , DOI 10.1016/j.str.2017.09.010 ,
Related entities 1. Cytoplasmic Tail of HIV-1 gp41 protein, : 1 : 58 entities Detail
Interaction partners 1. Cytoplasmic Tail of HIV-1 gp41 protein, : 1 interactors Detail
Experiments performed 14 experiments Detail
NMR combined restraints 5 contents Detail
Keywords HIV-1, VIRAL PROTEIN, alpha-helix, cytoplasmic tail, envelope, plasma membrane