BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	84.5 % (970 of 1148)	85.6 % (511 of 597)	80.8 % (366 of 453)	94.9 % (93 of 98)
Backbone	93.6 % (515 of 550)	92.5 % (173 of 187)	94.2 % (258 of 274)	94.4 % (84 of 89)
Sidechain	79.0 % (542 of 686)	82.4 % (338 of 410)	73.0 % (195 of 267)	100.0 % (9 of 9)
Aromatic	35.1 % (40 of 114)	63.2 % (36 of 57)	3.6 % (2 of 55)	100.0 % (2 of 2)
Methyl	93.9 % (107 of 114)	94.7 % (54 of 57)	93.0 % (53 of 57)

1. entity 1

GHMSSKIFKN CVIYINGYTK PGRLQLHEMI VLHGGKFLHY LSSKKTVTHI VASNLPLKKR IEFANYKVVS PDWIVDSVKE ARLLPWQNYS LTS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 15N] Rev1-BRCT, 50 mM Sodium phosphate buffer, 50 mM NaCl, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	NaCl	natural abundance	50 mM
2	Rev1-BRCT	[U-100% 15N]	1 mM
3	Sodium phosphate buffer	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] Rev1-BRCT, 50 mM sodium phosphate buffer, 50 mM NaCl, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.26 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.26 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.76 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5VX7, Strand ID: A Detail

Release date

2017-06-01

Citation

NMR mapping of PCNA interaction with translesion synthesis DNA polymerase Rev1 mediated by Rev1-BRCT domain
Xu, C., Cui, G., Botuyan, M.V., Mer, G.
J. Mol. Biol. (2013), 425, 3091-3105, PubMed 23747975 , DOI 10.1016/j.jmb.2013.05.029 , Abstract

Related entities 1. DNA repair protein REV1 (E.C.2.7.7.-), : 1 : 1 : 4 : 138 entities Detail

Interaction partners 1. DNA repair protein REV1 (E.C.2.7.7.-), : 10 interactors Detail

More details for 10 interactors identified by 7 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 15N] Rev1-BRCT, 50 mM Sodium phosphate buffer, 50 mM NaCl, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	NaCl	natural abundance	50 mM
2	Rev1-BRCT	[U-100% 15N]	1 mM
3	Sodium phosphate buffer	natural abundance	50 mM

2 3D 1H-15N TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	NaCl	natural abundance	50 mM
2	Rev1-BRCT	[U-100% 15N]	1 mM
3	Sodium phosphate buffer	natural abundance	50 mM

3 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	NaCl	natural abundance	50 mM
2	Rev1-BRCT	[U-100% 15N]	1 mM
3	Sodium phosphate buffer	natural abundance	50 mM

4 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] Rev1-BRCT, 50 mM sodium phosphate buffer, 50 mM NaCl, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

5 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

6 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

7 3D HNCACB

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

8 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

9 3D HNCA

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

10 3D HN(CO)CA

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

11 3D HNCO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

12 3D H(CCO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

13 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

14 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

15 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

16 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	NaCl	natural abundance	50 mM
5	Rev1-BRCT	[U-100% 13C; U-100% 15N]	1 mM
6	sodium phosphate buffer	natural abundance	50 mM

nullKeywords BRCT domain, DNA damage response, DNA replication, Protein binding, TRANSFERASE, Translesion DNA synthesis

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.918 seconds)

BMRB: 30298

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. DNA repair protein REV1 (E.C.2.7.7.-), : 1 : 1 : 4 : 138 entities Detail

Interaction partners 1. DNA repair protein REV1 (E.C.2.7.7.-), : 10 interactors Detail

Experiments performed 16 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample