BMRBj - BMREntryMaster

Found 1 Document (0.874 seconds)

BMRB: 30300

5VZM

Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin

Authors

Cui, G., Botuyan, M.V., Mer, G.

Assembly

Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-)

Entity

1. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 1 (polymer, Thiol state: all disulfide bound), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 2 (polymer, Thiol state: all disulfide bound), 111 monomers, 11976.28 Da Detail

GHMPSPSQLD QSVLEALPPD LREQVEQVCA VQQAESHGDK KKEPVNGCNT GILPQPVGTV LLQIPEPQES NSDAGINLIA LPAFSQVDPE VFAALPAELQ RELKAAYDQR Q

Total weight

20541.012 Da

Max. entity weight

11976.28 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	2:CYS29:SG	2:CYS48:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 94.4 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.4 % (2043 of 2165)	93.3 % (1062 of 1138)	97.0 % (805 of 830)	89.3 % (176 of 197)
Backbone	96.9 % (1058 of 1092)	96.5 % (358 of 371)	97.3 % (534 of 549)	96.5 % (166 of 172)
Sidechain	92.7 % (1157 of 1248)	91.8 % (704 of 767)	97.1 % (443 of 456)	40.0 % (10 of 25)
Aromatic	82.4 % (56 of 68)	82.4 % (28 of 34)	82.4 % (28 of 34)
Methyl	99.6 % (223 of 224)	99.1 % (111 of 112)	100.0 % (112 of 112)

1. entity 1

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. entity 2

GHMPSPSQLD QSVLEALPPD LREQVEQVCA VQQAESHGDK KKEPVNGCNT GILPQPVGTV LLQIPEPQES NSDAGINLIA LPAFSQVDPE VFAALPAELQ RELKAAYDQR Q

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 20 mM Sodium phosphate buffer, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 1 mM [U-100% 13C; U-100% 15N] Rev1, 3 mM Ubiquitin, 50 mM Sodium phosphate buffer, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 3 mM Rev1, 1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 50 mM Sodium phosphate buffer, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	Rev1	natural abundance	3 mM
7	Sodium phosphate buffer	natural abundance	50 mM
8	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Release date

2017-05-31

Citation

Structural Basis for the Interaction of Mutasome Assembly Factor REV1 with Ubiquitin
Cui, G., Botuyan, M.V., Mer, G.
J. Mol. Biol. (2018), 430, 2042-2050, PubMed 29778604 , DOI 10.1016/j.jmb.2018.05.017 , Abstract

Related entities 1. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 1, : 92 : 6 : 47 : 182 entities Detail

Related entities 2. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 2, : 1 : 14 entities Detail

Interaction partners 2. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 36 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 20 mM Sodium phosphate buffer, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

2 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

4 3D HNCACB

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

5 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

6 3D HNCO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

7 3D HN(CA)CO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

8 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

9 3D H(CCO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

10 3D (H)CC(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

11 3D 1H-15N TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

12 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

13 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

14 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

15 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 1 mM [U-100% 13C; U-100% 15N] Rev1, 3 mM Ubiquitin, 50 mM Sodium phosphate buffer, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

16 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

17 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

18 3D HNCACB

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

19 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

20 3D HNCO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

21 3D HN(CA)CO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

22 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

23 3D H(CCO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

24 3D (H)CC(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

25 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

26 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Sodium phosphate buffer	natural abundance		20 mM
2	Ubiquitin	[U-100% 13C; U-100% 15N]		1 mM

27 3D 1H-15N TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

28 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

29 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

30 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

31 13C,15N-filetered/edited NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
3	Rev1	[U-100% 13C; U-100% 15N]	1 mM
4	Sodium phosphate buffer	natural abundance	50 mM
5	Ubiquitin	natural abundance	3 mM

32 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 3 mM Rev1, 1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 50 mM Sodium phosphate buffer, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	Rev1	natural abundance	3 mM
7	Sodium phosphate buffer	natural abundance	50 mM
8	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM

33 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	Rev1	natural abundance	3 mM
7	Sodium phosphate buffer	natural abundance	50 mM
8	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM

34 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	Rev1	natural abundance	3 mM
7	Sodium phosphate buffer	natural abundance	50 mM
8	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM

35 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	Rev1	natural abundance	3 mM
7	Sodium phosphate buffer	natural abundance	50 mM
8	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM

36 13C,15N-filetered/edited NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	Rev1	natural abundance	3 mM
7	Sodium phosphate buffer	natural abundance	50 mM
8	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM

nullKeywords DNA repair, DNA replication, PCNA, PROTEIN BINDING, Rev1, Translesion DNA synthesis, ubiquitin

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.874 seconds)

BMRB: 30300

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 1, : 92 : 6 : 47 : 182 entities Detail

Related entities 2. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 2, : 1 : 14 entities Detail

Interaction partners 2. Ubiquitin, DNA repair protein REV1 (E.C.2.7.7.-), entity 2, : 1 interactors Detail

Experiments performed 36 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample