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BMRB: 30303

5W3G

Solution Structure of ETS Transcription Factor PU.1

Authors

Lau, D.K.W., Okon, M., McIntosh, L.P.

Assembly

Transcription factor PU.1

Entity

1. Transcription factor PU.1 (polymer, Thiol state: not present), 110 monomers, 12899.97 Da Detail

HIHMGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY QFSGEVLGRG GLAERRLPPH

Formula weight

12899.97 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 84.7 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.7 % (1176 of 1388)	80.1 % (593 of 740)	88.7 % (470 of 530)	95.8 % (113 of 118)
Backbone	95.6 % (627 of 656)	93.9 % (215 of 229)	96.9 % (309 of 319)	95.4 % (103 of 108)
Sidechain	77.6 % (645 of 831)	74.0 % (378 of 511)	82.9 % (257 of 310)	100.0 % (10 of 10)
Aromatic	59.4 % (76 of 128)	60.9 % (39 of 64)	55.7 % (34 of 61)	100.0 % (3 of 3)
Methyl	95.7 % (90 of 94)	93.6 % (44 of 47)	97.9 % (46 of 47)

1. Transcription factor PU.1

HIHMGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY QFSGEVLGRG GLAERRLPPH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.3 mM [U-99% 13C; U-99% 15N] DNA binding protein, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA binding protein	[U-99% 13C; U-99% 15N]		0.3 mM
2	potassium chloride	natural abundance		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 2 Detail

Release date

2017-06-20

Citation

The Biophysical Basis for Phosphorylation-Enhanced DNA-Binding Autoinhibition of the ETS1 Transcription Factor
Perez-Borrajero, C., Lin, C.S., Okon, M., Scheu, K., Graves, B.J., Murphy, M., McIntosh, L.P.
J. Mol. Biol. (2018), 431, 593-614, PubMed 30597162 , DOI 10.1016/j.jmb.2018.12.011 , Abstract

Related entities 1. Transcription factor PU.1, : 1 : 36 : 110 entities Detail

Interaction partners 1. Transcription factor PU.1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC