NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra
SEQFTTKLNT LEDSQESISS ASKWLLLQYR DAPKVAEMWK EYMLRPSVNT RRKLLGLYLM NHVVQQAKGQ KIIQFQDSFG KVAAEVLGRI NQEFPRDLKK KLSRVVNILK ERNIFSKQVV NDIERSLAAA LEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.7 % (1442 of 1703) | 90.4 % (810 of 896) | 74.7 % (488 of 653) | 93.5 % (144 of 154) |
Backbone | 82.6 % (679 of 822) | 94.6 % (262 of 277) | 70.7 % (290 of 410) | 94.1 % (127 of 135) |
Sidechain | 87.9 % (892 of 1015) | 88.5 % (548 of 619) | 86.7 % (327 of 377) | 89.5 % (17 of 19) |
Aromatic | 27.0 % (34 of 126) | 27.0 % (17 of 63) | 27.9 % (17 of 61) | 0.0 % (0 of 2) |
Methyl | 99.4 % (157 of 158) | 98.7 % (78 of 79) | 100.0 % (79 of 79) |
1. Regulator of Ty1 transposition protein 103
SEQFTTKLNT LEDSQESISS ASKWLLLQYR DAPKVAEMWK EYMLRPSVNT RRKLLGLYLM NHVVQQAKGQ KIIQFQDSFG KVAAEVLGRI NQEFPRDLKK KLSRVVNILK ERNIFSKQVV NDIERSLAAA LEHHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.8 mM [U-13C; U-15N] Rtt103 (RTT) protein, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Rtt103 (RTT) protein | [U-13C; U-15N] | 0.8 mM | |
2 | Potassium Phosphate | natural abundance | 35 mM | |
3 | KCl | natural abundance | 100 mM |