BMRB: 30371

NMR Structural and biophysical functional analysis of intracellular loop 5 of the NHE1 isoform of the Na+/H+ exchanger.

Authors

McKay, R., Wong, K., Towle, K., Fliegel, L.

Assembly

GLY-LEU-THR-TRP-PHE-ILE-ASN-LYS-PHE-ARG-ILE-VAL-LYS

Entity

1. GLY-LEU-THR-TRP-PHE-ILE-ASN-LYS-PHE-ARG-ILE-VAL-LYS (polymer, Thiol state: not present), 13 monomers, 1621.964 Da Detail

GLTWFINKFR IVK

Formula weight

1621.964 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chemcal Shifts

¹H: 97 assignments Detail

Download

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	GLY	H	H	8.1290	0.03	1
2	1	1	GLY	HA2	H	3.9120	0.03	1
3	1	1	GLY	HA3	H	4.0310	0.03	1
4	1	2	LEU	H	H	8.4030	0.03	1
5	1	2	LEU	HA	H	4.3510	0.03	1
6	1	2	LEU	HB2	H	1.8170	0.03	1
7	1	2	LEU	HD11	H	0.9670	0.03	1
8	1	2	LEU	HD12	H	0.9670	0.03	1
9	1	2	LEU	HD13	H	0.9670	0.03	1
10	1	2	LEU	HD21	H	1.0230	0.03	1
11	1	2	LEU	HD22	H	1.0230	0.03	1
12	1	2	LEU	HD23	H	1.0230	0.03	1
13	1	3	THR	H	H	8.0580	0.03	1
14	1	3	THR	HA	H	3.9240	0.03	1
15	1	3	THR	HB	H	4.2370	0.03	1
16	1	3	THR	HG21	H	1.3130	0.03	1
17	1	3	THR	HG22	H	1.3130	0.03	1
18	1	3	THR	HG23	H	1.3130	0.03	1
19	1	4	TRP	H	H	7.9030	0.03	1
20	1	4	TRP	HA	H	4.3620	0.03	1
21	1	4	TRP	HB2	H	3.4440	0.03	1
22	1	4	TRP	HB3	H	3.3090	0.03	1
23	1	4	TRP	HD1	H	7.2680	0.03	1
24	1	4	TRP	HE1	H	9.9040	0.03	1
25	1	4	TRP	HE3	H	7.4590	0.03	1
26	1	4	TRP	HZ2	H	7.4510	0.03	1
27	1	4	TRP	HZ3	H	6.8080	0.03	1
28	1	4	TRP	HH2	H	7.0440	0.03	1
29	1	5	PHE	H	H	8.0380	0.03	1
30	1	5	PHE	HA	H	3.8920	0.03	1
31	1	5	PHE	HB2	H	3.2870	0.03	1
32	1	5	PHE	HB3	H	3.2270	0.03	1
33	1	5	PHE	HD1	H	7.2860	0.03	1
34	1	5	PHE	HD2	H	7.2870	0.03	1
35	1	5	PHE	HE1	H	7.1230	0.03	1
36	1	6	ILE	H	H	8.3740	0.03	1
37	1	6	ILE	HA	H	3.6240	0.03	1
38	1	6	ILE	HB	H	1.9350	0.03	1
39	1	6	ILE	HG12	H	1.2480	0.03	1
40	1	6	ILE	HG13	H	1.7970	0.03	1
41	1	6	ILE	HG21	H	0.9670	0.03	1
42	1	6	ILE	HG22	H	0.9670	0.03	1
43	1	6	ILE	HG23	H	0.9670	0.03	1
44	1	6	ILE	HD11	H	0.8940	0.03	1
45	1	6	ILE	HD12	H	0.8940	0.03	1
46	1	6	ILE	HD13	H	0.8940	0.03	1
47	1	7	ASN	H	H	7.9760	0.03	1
48	1	7	ASN	HA	H	4.3690	0.03	1
49	1	7	ASN	HB2	H	2.8150	0.03	1
50	1	7	ASN	HB3	H	2.6370	0.03	1
51	1	8	LYS	H	H	7.6340	0.03	1
52	1	8	LYS	HA	H	3.8250	0.03	1
53	1	8	LYS	HB2	H	1.3090	0.03	1
54	1	8	LYS	HG2	H	0.6030	0.03	1
55	1	8	LYS	HD2	H	0.8270	0.03	1
56	1	8	LYS	HE2	H	2.4600	0.03	1
57	1	8	LYS	HE3	H	2.6740	0.03	1
58	1	9	PHE	H	H	8.1910	0.03	1
59	1	9	PHE	HA	H	4.0420	0.03	1
60	1	9	PHE	HB2	H	2.7980	0.03	1
61	1	9	PHE	HB3	H	3.1370	0.03	1
62	1	9	PHE	HD1	H	7.1070	0.03	1
63	1	9	PHE	HE1	H	7.0390	0.03	1
64	1	10	ARG	H	H	8.0280	0.03	1
65	1	10	ARG	HA	H	3.8800	0.03	1
66	1	10	ARG	HB2	H	1.9110	0.03	1
67	1	10	ARG	HG2	H	1.6890	0.03	1
68	1	10	ARG	HD2	H	3.1890	0.03	1
69	1	10	ARG	HE	H	7.0300	0.03	1
70	1	10	ARG	HH12	H	7.4520	0.03	1
71	1	10	ARG	HH21	H	6.8100	0.03	1
72	1	11	ILE	H	H	7.5590	0.03	1
73	1	11	ILE	HA	H	3.8450	0.03	1
74	1	11	ILE	HB	H	1.9350	0.03	1
75	1	11	ILE	HG12	H	1.1790	0.03	1
76	1	11	ILE	HG21	H	0.8740	0.03	1
77	1	11	ILE	HG22	H	0.8740	0.03	1
78	1	11	ILE	HG23	H	0.8740	0.03	1
79	1	11	ILE	HD11	H	0.7970	0.03	1
80	1	11	ILE	HD12	H	0.7970	0.03	1
81	1	11	ILE	HD13	H	0.7970	0.03	1
82	1	12	VAL	H	H	7.7000	0.03	1
83	1	12	VAL	HA	H	3.8450	0.03	1
84	1	12	VAL	HB	H	2.0420	0.03	1
85	1	12	VAL	HG11	H	0.8980	0.03	1
86	1	12	VAL	HG12	H	0.8980	0.03	1
87	1	12	VAL	HG13	H	0.8980	0.03	1
88	1	12	VAL	HG21	H	0.9700	0.03	1
89	1	12	VAL	HG22	H	0.9700	0.03	1
90	1	12	VAL	HG23	H	0.9700	0.03	1
91	1	13	LYS	H	H	7.8340	0.03	1
92	1	13	LYS	HA	H	4.0780	0.03	1
93	1	13	LYS	HB2	H	1.9530	0.03	1
94	1	13	LYS	HG2	H	1.4240	0.03	1
95	1	13	LYS	HG3	H	1.3450	0.03	1
96	1	13	LYS	HD2	H	1.5820	0.03	1
97	1	13	LYS	HE2	H	2.9490	0.03	1

Release date

2018-01-24

Citation

Diverse residues of intracellular loop 5 of the Na⁺/H⁺ exchanger modulate proton sensing, expression, activity and targeting
Wong, K.Y., McKay, R., Liu, Y., Towle, K., Elloumi, Y., Li, X., Quan, S., Dutta, D., Sykes, B.D., Fliegel, L.
Biochim. Biophys. Acta (2018), 1861, 191-200, PubMed 30071192 , DOI 10.1016/j.bbamem.2018.07.014 , Abstract

Related entities 1. GLY-LEU-THR-TRP-PHE-ILE-ASN-LYS-PHE-ARG-ILE-VAL-LYS, : 5 : 5 : 1 entities Detail

Interaction partners 1. GLY-LEU-THR-TRP-PHE-ILE-ASN-LYS-PHE-ARG-ILE-VAL-LYS, : 13 interactors Detail

Experiments performed 4 experiments Detail