BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.3 % (1224 of 1355)	90.7 % (643 of 709)	89.6 % (476 of 531)	91.3 % (105 of 115)
Backbone	92.6 % (626 of 676)	91.8 % (212 of 231)	93.8 % (316 of 337)	90.7 % (98 of 108)
Sidechain	88.7 % (697 of 786)	90.2 % (431 of 478)	86.4 % (260 of 301)	85.7 % (6 of 7)
Aromatic	62.1 % (87 of 140)	70.0 % (49 of 70)	53.6 % (37 of 69)	100.0 % (1 of 1)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. entity 1

MHHHHHHSTS VDLGTENLYF QSNAGKMFIG GLSWQTSPDS LRDYFSKFGE IRECMVMRDP TTKRSRGFGF VTFADPASVD KVLGQPHHEL DSKTIDPKVA FPRRAQPKMV TRTKK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MSI2-RRM1	[U-90% 15N]		0.5 mM
2	NaCl	natural abundance		150 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.7 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.6 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 1 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.4 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 6C8U, Strand ID: 1 Detail

Release date

2018-08-14

Citation

Crystal and solution structures of human oncoprotein Musashi-2 N-terminal RNA recognition motif 1
Lan, L., Xing, M., Kashipathy, M., Douglas, J., Gao, P., Battaile, K., Hanzlik, R., Lovell, S., Xu, L.
Proteins (2020), 88, 573-583, PubMed 31603583 , DOI 10.1002/prot.25836 , Abstract

Related entities 1. RNA-binding protein Musashi homolog 2, : 25 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MSI2-RRM1	[U-90% 15N]		0.5 mM
2	NaCl	natural abundance		150 mM

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.7 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.6 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 1 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.4 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

3 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

5 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

6 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

7 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

8 3D HNCACO

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

11 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MSI2-RRM1	[U-90% 15N]		0.5 mM
2	NaCl	natural abundance		150 mM

12 3D HCCH-COSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_30398_6c8u.nef
Input source #2: Coordindates	6c8u.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): 1 vs Auth_asym_ID (model): 1

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
1	1	115	0	0	100.0

Content subtype: combined_30398_6c8u.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1226		93.9 (chain: 1, length: 115)
1	Distance restraints	CNS/XPLOR_distance_constraints_5	OK	977 (1)	noe	91.3 (chain: 1, length: 115)
2	Distance restraints	CNS/XPLOR_distance_constraints_7	OK	639 (1)	noe	78.3 (chain: 1, length: 115)
3	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	38 (1)	hbond	27.8 (chain: 1, length: 115)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_6	OK	148 (148)	.	73.0 (chain: 1, length: 115)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 93.9%
- Total number of assignments
  - ¹H chemical shifts: 642
  - ¹³C chemical shifts: 477
  - ¹⁵N chemical shifts: 107
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
13	ASN	CG	176.73
19	ASN	CG	176.58
31	GLN	CD	180.25
81	GLN	CD	180.44
102	GLN	CD	180.46

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	709	642	90.6
¹³C chemical shifts	531	472	88.9
¹⁵N chemical shifts	123	107	87.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	231	214	92.6
¹³C chemical shifts	230	216	93.9
¹⁵N chemical shifts	108	98	90.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	478	428	89.5
¹³C chemical shifts	301	256	85.0
¹⁵N chemical shifts	15	9	60.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	50	98.0
¹³C chemical shifts	51	50	98.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	45	64.3
¹³C chemical shifts	69	33	47.8
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_5
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 91.3%
- Total number of restraints: 975
- Weight of restraints: 1.0 (975)
- Potential type of restraints: square-well-parabolic (975)
- Range of distance target values: 2.1, 3.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: 1
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_7
  - Status: OK
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 78.3%
  - Total number of restraints: 637
  - Weight of restraints: 1.0 (637)
  - Potential type of restraints: square-well-parabolic (637)
  - Range of distance target values: 2.1, 3.5 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: 1
  - Distance restraints on contact map
  - Saveframe: CNS/XPLOR_distance_constraints_4
    - Status: Warning
    - Experiment type: hbond
    - Sequence coverage of experimental data
      - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 27.8%
    - Total number of restraints: 38
    - Weight of restraints: 1.0 (38)
    - Potential type of restraints: square-well-parabolic (38)
    - Range of distance target values: 2.05, 2.95 (Å)
    - Histogram of distance target values
    - Distance restraints per residue
      - Chain_ID: 1
    - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_6
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 73.0%
- Total number of restraints: 148
- Total number of restraints per polymer type: 148
- Weight of restraints: 1.0 (148)
- Potential type of restraints: square-well-parabolic (148)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: 1

Keywords RNA binding protein, RNA recognition motif

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Found 1 Document (0.623 seconds)

BMRB: 30398

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA-binding protein Musashi homolog 2, : 25 entities Detail

Experiments performed 12 experiments Detail

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NMR combined restraints 6 contents Detail