NMR data-driven model of GTPase KRas-GMPPNP:Cmpd2 complex tethered to a nanodisc
GPLKLLDNWD SVTSTFSKLR EQLGPVTQEF WDNLEKETEG LRQEMSKDLE EVKAKVQPYL DDFQKKWQEE MELYRQKVEP LRAELQEGAR QKLHELQEKL SPLGEEMRDR ARAHVDALRT HLAPYSDELR QRLAARLEAL KENGGARLAE YHAKATEHLS TLSEKAKPAL EDLRQGLLPV LESFKVSFLS ALEEYTKKLN
GSMTEYKLVV VGAVGVGKSA LTIQLIQNHF VDEYDPTIED SYRKQVVIDG ETCLLDILDT AGQEEYSAMR DQYMRTGEGF LCVFAINNTK SFEDIHHYRE QIKRVKDSED VPMVLVGNKC DLPSRTVDTK QAQDLARSYG IPFIETSAKT RQGVDDAFYT LVREIRKHKE KMSKDGKKKK KKSKTKC
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 7.9 % (363 of 4619) | 7.5 % (183 of 2430) | 4.8 % (85 of 1782) | 23.3 % (95 of 407) |
Backbone | 10.7 % (246 of 2298) | 13.8 % (108 of 782) | 3.9 % (44 of 1141) | 25.1 % (94 of 375) |
Sidechain | 4.9 % (133 of 2688) | 4.6 % (75 of 1648) | 5.7 % (57 of 1008) | 3.1 % (1 of 32) |
Aromatic | 1.4 % (4 of 286) | 1.4 % (2 of 143) | 1.4 % (2 of 140) | 0.0 % (0 of 3) |
Methyl | 14.6 % (60 of 410) | 14.6 % (30 of 205) | 14.6 % (30 of 205) |
1. entity 1
GPLKLLDNWD SVTSTFSKLR EQLGPVTQEF WDNLEKETEG LRQEMSKDLE EVKAKVQPYL DDFQKKWQEE MELYRQKVEP LRAELQEGAR QKLHELQEKL SPLGEEMRDR ARAHVDALRT HLAPYSDELR QRLAARLEAL KENGGARLAE YHAKATEHLS TLSEKAKPAL EDLRQGLLPV LESFKVSFLS ALEEYTKKLN2. entity 2
GSMTEYKLVV VGAVGVGKSA LTIQLIQNHF VDEYDPTIED SYRKQVVIDG ETCLLDILDT AGQEEYSAMR DQYMRTGEGF LCVFAINNTK SFEDIHHYRE QIKRVKDSED VPMVLVGNKC DLPSRTVDTK QAQDLARSYG IPFIETSAKT RQGVDDAFYT LVREIRKHKE KMSKDGKKKK KKSKTKCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.2 mM U-15N, Ile, Leu C-delta-13C, Val C-gamma-13C GTPase KRas isoform b, 0.4 mM Membrane Scaffold Protein, 100 mM sodium chloride, 20 mM TRIS, 5 mM magnesium chloride, 2 mM TCEP, 0.2 mM GMPPNP, 12 mM DOPC, 3.2 mM DOPS, 0.8 mM PE-MCC, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | GTPase KRas isoform b | U-15N, Ile, Leu C-delta-13C, Val C-gamma-13C | 0.2 mM | |
2 | Membrane Scaffold Protein | natural abundance | 0.4 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | TRIS | natural abundance | 20 mM | |
5 | magnesium chloride | natural abundance | 5 mM | |
6 | TCEP | natural abundance | 2 mM | |
7 | GMPPNP | natural abundance | 0.2 mM | |
8 | DOPC | natural abundance | 12 mM | |
9 | DOPS | natural abundance | 3.2 mM | |
10 | PE-MCC | natural abundance | 0.8 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.2 mM GTPase KRas isoform b, 0.4 mM Membrane Scaffold Protein, 100 mM sodium chloride, 20 mM TRIS, 5 mM magnesium chloride, 2 mM TCEP, 0.2 mM GMPPNP, 12 mM DOPC, 3.2 mM DOPS, 0.8 mM PE-MCC, 0.4 mM PE-DTPA-Gd, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | GTPase KRas isoform b | natural abundance | 0.2 mM | |
12 | Membrane Scaffold Protein | natural abundance | 0.4 mM | |
13 | sodium chloride | natural abundance | 100 mM | |
14 | TRIS | natural abundance | 20 mM | |
15 | magnesium chloride | natural abundance | 5 mM | |
16 | TCEP | natural abundance | 2 mM | |
17 | GMPPNP | natural abundance | 0.2 mM | |
18 | DOPC | natural abundance | 12 mM | |
19 | DOPS | natural abundance | 3.2 mM | |
20 | PE-MCC | natural abundance | 0.8 mM | |
21 | PE-DTPA-Gd | natural abundance | 0.4 mM |