BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	70.0 % (1004 of 1435)	74.1 % (559 of 754)	62.0 % (346 of 558)	80.5 % (99 of 123)
Backbone	86.6 % (608 of 702)	83.7 % (201 of 240)	88.3 % (308 of 349)	87.6 % (99 of 113)
Sidechain	58.8 % (496 of 844)	69.6 % (358 of 514)	43.1 % (138 of 320)	0.0 % (0 of 10)
Aromatic	12.1 % (14 of 116)	20.7 % (12 of 58)	3.5 % (2 of 57)	0.0 % (0 of 1)
Methyl	56.3 % (71 of 126)	85.7 % (54 of 63)	27.0 % (17 of 63)

1. Titin

RMAHEGALTG VTTDQKEKQK PDIVLYPEPV RVLEGETARF RCRVTGYPQP KVNWYLNGQL IRKSKRFRVR YDGIHYLDIV DCKSYDTGEV KVTAENPEGV IEHKVKLEIQ QLEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 20 mM TRIS, 50 mM sodium chloride, 1 mM [U-99% 13C; U-99% 15N] Human Titin ZIg10, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6DL4, Strand ID: A Detail

Release date

2018-07-23

Citation

Structural Insights on the Obscurin-Binding Domains in Titin
Letourneau, A.G., Wright, N.T.
Protein Pept. Lett. (2018), 25, 973-979, PubMed 30289063 , DOI 10.2174/0929866525666181004102031 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30514 released on 2019-01-08
    Title Human Obscurin Ig57 Domain
  BMRB: 27497 released on 2018-05-31
    Title Human titin ZIg10

Related entities 1. Titin (E.C.2.7.11.1), : 1 : 1 : 228 entities Detail

Interaction partners 1. Titin (E.C.2.7.11.1), : 55 interactors Detail

More details for 55 interactors identified by 26 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 20 mM TRIS, 50 mM sodium chloride, 1 mM [U-99% 13C; U-99% 15N] Human Titin ZIg10, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

4 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

5 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

6 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

7 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

8 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	Human Titin ZIg10	[U-99% 13C; U-99% 15N]	1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30471_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30471_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1016		88.2 (chain: 1, length: 119)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 119, Sequence coverage: 88.2%
- Total number of assignments
  - ¹H chemical shifts: 623
  - ¹³C chemical shifts: 299
  - ¹⁵N chemical shifts: 94
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
75	HIS	HD1	7.03

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	754	514	68.2
¹³C chemical shifts	558	299	53.6
¹⁵N chemical shifts	132	94	71.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	240	192	80.0
¹³C chemical shifts	238	202	84.9
¹⁵N chemical shifts	113	94	83.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	514	322	62.6
¹³C chemical shifts	320	97	30.3
¹⁵N chemical shifts	19	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	54	84.4
¹³C chemical shifts	64	3	4.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	11	19.0
¹³C chemical shifts	57	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords STRUCTURAL PROTEIN, Titin, cytoskeleton, sarcomere

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.046 seconds)

BMRB: 30471

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Titin (E.C.2.7.11.1), : 1 : 1 : 228 entities Detail

Interaction partners 1. Titin (E.C.2.7.11.1), : 55 interactors Detail

Experiments performed 10 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail