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Found 1 Document (0.885 seconds)

BMRB: 30472

6DLN

Oligomeric Structure of the HIV gp41 MPER-TMD in Phospholipid Bilayers

Authors

Kwon, B., Lee, M., Waring, A.J., Hong, M.

Assembly

Transmembrane protein gp41

Entity

1. Transmembrane protein gp41 (polymer, Thiol state: not present), 39 monomers, 4669.620 × 3 Da Detail

KWASLWNWFN ITNWLWYIKL FIMIVGGLVG LRIVFAVLS

Total weight

14008.86 Da

Max. entity weight

4669.62 Da

Source organism

HIV-1 M:B_HXB2R

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 12.8 %, Completeness: 11.0 %, Completeness (bb): 12.3 % Detail

Polymer type: polypeptide(L)

	Total	¹³C
All	11.0 % (23 of 210)	11.0 % (23 of 210)
Backbone	12.3 % (14 of 114)	12.3 % (14 of 114)
Sidechain	9.8 % (13 of 132)	9.8 % (13 of 132)
Aromatic	0.0 % (0 of 44)	0.0 % (0 of 44)
Methyl	21.2 % (7 of 33)	21.2 % (7 of 33)

1. entity 1

KWASLWNWFN ITNWLWYIKL FIMIVGGLVG LRIVFAVLS

Show sample condition

Sample #1

Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 233 K, pH 7.5, Details 33 % w/w 4-19F-F699 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	HIV gp41 MPER-TMD	4-19F-F699		33 (±5.0) % w/w
2	HEPES buffer	natural abundance		10 mM

Sample #2

Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 233 K, pH 7.5, Details 33 % w/w [U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	HIV gp41 MPER-TMD	[U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699		33 (±5.0) % w/w
4	HEPES buffer	natural abundance		10 mM

Sample #3

Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 233 K, pH 7.5, Details 33 % w/w [U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	HIV gp41 MPER-TMD	[U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680		33 (±5.0) % w/w
6	HEPES buffer	natural abundance		10 mM

Release date

2018-06-25

Citation

Oligomeric Structure and Three-Dimensional Fold of the HIV gp41 Membrane-Proximal External Region and Transmembrane Domain in Phospholipid Bilayers
Kwon, B., Lee, M., Waring, A.J., Hong, M.
J. Am. Chem. Soc. (2018), 140, 8246-8259, PubMed 29888593 , DOI 10.1021/jacs.8b04010 , Abstract

Related entities 1. Transmembrane protein gp41, : 1 : 6 : 17 : 75 entities Detail

Interaction partners 1. Transmembrane protein gp41, : 8 interactors Detail

More details for 8 interactors identified by 5 citations

Experiments performed 7 experiments Detail

1 2D 13C-13C DARR

Instrument

Bruker Bruker - 800 MHz

Sample

State isotropic, Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 263 K, pH 7.5, Details 33 % w/w [U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	HIV gp41 MPER-TMD	[U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680		33 (±5.0) % w/w
6	HEPES buffer	natural abundance		10 mM

2 2D water Edited DARR

Instrument

Bruker Bruker - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
5	HIV gp41 MPER-TMD	[U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680		33 (±5.0) % w/w
6	HEPES buffer	natural abundance		10 mM

3 13C-19F REDOR

Instrument

Bruker Bruker - 400 MHz

Sample

State isotropic, Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 233 K, pH 7.5, Details 33 % w/w [U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	HIV gp41 MPER-TMD	[U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699		33 (±5.0) % w/w
4	HEPES buffer	natural abundance		10 mM

4 2D water Edited DARR

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 263 K, pH 7.5, Details 33 % w/w [U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	HIV gp41 MPER-TMD	[U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699		33 (±5.0) % w/w
4	HEPES buffer	natural abundance		10 mM

5 19F CODEX

Instrument

Bruker Bruker - 400 MHz

Sample

State isotropic, Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 233 K, pH 7.5, Details 33 % w/w 4-19F-F699 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	HIV gp41 MPER-TMD	4-19F-F699		33 (±5.0) % w/w
2	HEPES buffer	natural abundance		10 mM

6 19F CODEX

Instrument

Bruker Bruker - 400 MHz

Sample

State isotropic, Solvent system 10 mM HEPES buffer pH 7.5, Pressure 1 atm, Temperature 233 K, pH 7.5, Details 33 % w/w [U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680 HIV gp41 MPER-TMD, reconstituted into the virus mimetic membrane (POPC:POPE:POPS:sphingomyelin: cholesterol = 30:15:15:10:30), 10 mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	HIV gp41 MPER-TMD	[U-13C; U-15N]-L669,I686, A700, 13C'-G694, 19F-5F-W680		33 (±5.0) % w/w
6	HEPES buffer	natural abundance		10 mM

7 19F CODEX

Instrument

Bruker Bruker - 400 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	HIV gp41 MPER-TMD	[U-13C; U-15N]-L684,I686, G694, 19F-5F-W678, 19F-4F-F699		33 (±5.0) % w/w
4	HEPES buffer	natural abundance		10 mM

nullKeywords HIV, MEMBRANE PROTEIN, MPER-TMD

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Found 1 Document (0.885 seconds)

BMRB: 30472

Sample #1

Sample #2

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Related entities 1. Transmembrane protein gp41, : 1 : 6 : 17 : 75 entities Detail

Interaction partners 1. Transmembrane protein gp41, : 8 interactors Detail

Experiments performed 7 experiments Detail

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