BMRBj - BMREntryMaster

Found 1 Document (4.629 seconds)

BMRB: 30500

6E5N

Structure of a protein complex

Authors

Buel, G.R., Walters, K.J.

Assembly

Unconventional myosin-VI, Clathrin light chain A

Entity

1. Unconventional myosin-VI, Clathrin light chain A, entity 1 (polymer), 87 monomers, 9970.475 Da Detail

GPLGSRPKMT PEQMAKEMSE FLSRGPAVLA TKAAAGTKKY DLSKWKYAEL RDTINTSCDI ELLAACREEF HRRLKVYHAW KSKNKKR

2. Unconventional myosin-VI, Clathrin light chain A, entity 2 (polymer, Thiol state: not present), 24 monomers, 2373.527 Da Detail

GPLGSPEFIE NDEAFAILDG GAPG

Total weight

12344.002 Da

Max. entity weight

9970.475 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.1 %, Completeness: 88.8 %, Completeness (bb): 88.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.8 % (1148 of 1293)	89.9 % (612 of 681)	86.9 % (436 of 502)	90.9 % (100 of 110)
Backbone	88.3 % (576 of 652)	87.9 % (197 of 224)	88.0 % (285 of 324)	90.4 % (94 of 104)
Sidechain	89.6 % (666 of 743)	90.8 % (415 of 457)	87.5 % (245 of 280)	100.0 % (6 of 6)
Aromatic	75.0 % (72 of 96)	81.3 % (39 of 48)	67.4 % (31 of 46)	100.0 % (2 of 2)
Methyl	89.0 % (89 of 100)	88.0 % (44 of 50)	90.0 % (45 of 50)

1. entity 1

GPLGSRPKMT PEQMAKEMSE FLSRGPAVLA TKAAAGTKKY DLSKWKYAEL RDTINTSCDI ELLAACREEF HRRLKVYHAW KSKNKKR

2. entity 2

GPLGSPEFIE NDEAFAILDG GAPG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 760 mmHg, Temperature 283.2 K, pH 6.5, Details 0.36 mM [U-13C; U-15N] Myosin VI 1050-1131, 0.36 mM None Clathrin Light Chain Alpha 46-61, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 760 mmHg, Temperature 283.2 K, pH 6.5, Details 0.4 mM None Myosin VI 1050-1131, 0.4 mM [U-13C; U-15N] Clathrin Light Chain Alpha 46-61, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6E5N, Strand ID: A, B Detail

Release date

2018-07-23

Citation

Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension
Biancospino, M., Buel, G.R., Nino, C.A., Maspero, E., Scotto di Perrotolo, R., Raimondi, A., Redlingshofer, L., Weber, J., Brodsky, F.M., Walters, K.J., Polo, S.
Nat. Commun. (2019), 10, 4974-4974, PubMed 31672988 , DOI 10.1038/s41467-019-12855-6 , Abstract

Related entities 1. Unconventional myosin-VI, Clathrin light chain A, entity 1, : 1 : 1 : 10 entities Detail

Related entities 2. Unconventional myosin-VI, Clathrin light chain A, entity 2, : 1 : 5 entities Detail

Interaction partners 1. Unconventional myosin-VI, Clathrin light chain A, entity 1, : 74 interactors Detail

More details for 74 interactors identified by 22 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 760 mmHg, Temperature 283.2 K, pH 6.5, Details 0.36 mM [U-13C; U-15N] Myosin VI 1050-1131, 0.36 mM None Clathrin Light Chain Alpha 46-61, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

2 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

3 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

4 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

5 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 760 mmHg, Temperature 283.2 K, pH 6.5, Details 0.4 mM None Myosin VI 1050-1131, 0.4 mM [U-13C; U-15N] Clathrin Light Chain Alpha 46-61, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

6 3D HNCO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

7 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

8 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

9 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

10 3D HNCACB

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

11 3D HNCACB

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

12 3D HNCO

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

13 half-filtered 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Myosin VI 1050-1131	[U-13C; U-15N]	0.36 mM
2	Clathrin Light Chain Alpha 46-61	natural abundance	0.36 mM
3	NaCl	natural abundance	50 mM
4	NaPO4	natural abundance	20 mM

14 half-filtered 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Myosin VI 1050-1131	natural abundance	0.4 mM
6	Clathrin Light Chain Alpha 46-61	[U-13C; U-15N]	0.4 mM
7	NaCl	natural abundance	50 mM
8	NaPO4	natural abundance	20 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30500_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30500_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1251		94.3 (chain: 1, length: 87), 66.7 (chain: 2, length: 24)
1	Chemical shift references	chem_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 87, Sequence coverage: 94.3%
  - Entity_assembly_ID: 2, Chain length: 24, Sequence coverage: 66.7%
- Total number of assignments
  - ¹H chemical shifts: 713
  - ¹³C chemical shifts: 431
  - ¹⁵N chemical shifts: 107
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
10	THR	HG1	5.698
43	SER	HG	6.005
53	THR	HG1	5.372
56	THR	HG1	5.043
57	SER	HG	6.097
66	CYS	HG	2.252
67	ARG	HH21	6.732
67	ARG	HH22	7.228

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	554	527	95.1
¹³C chemical shifts	404	374	92.6
¹⁵N chemical shifts	95	91	95.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	174	163	93.7
¹³C chemical shifts	174	158	90.8
¹⁵N chemical shifts	83	79	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	380	364	95.8
¹³C chemical shifts	230	216	93.9
¹⁵N chemical shifts	12	12	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	40	95.2
¹³C chemical shifts	42	40	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	38	35	92.1
¹³C chemical shifts	36	30	83.3
¹⁵N chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	127	80	63.0
¹³C chemical shifts	98	57	58.2
¹⁵N chemical shifts	22	16	72.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	34	68.0
¹³C chemical shifts	48	30	62.5
¹⁵N chemical shifts	21	15	71.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	77	46	59.7
¹³C chemical shifts	50	27	54.0
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	9	81.8
¹³C chemical shifts	11	9	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	4	40.0
¹³C chemical shifts	10	1	10.0

Keywords Clathrin, Endocytosis, MOTOR PROTEIN, Myosin, Trafficking

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Found 1 Document (4.629 seconds)

BMRB: 30500

Sample #1

Sample #2

Related entities 1. Unconventional myosin-VI, Clathrin light chain A, entity 1, : 1 : 1 : 10 entities Detail

Related entities 2. Unconventional myosin-VI, Clathrin light chain A, entity 2, : 1 : 5 entities Detail

Interaction partners 1. Unconventional myosin-VI, Clathrin light chain A, entity 1, : 74 interactors Detail

Experiments performed 14 experiments Detail

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Chemical shift validation 4 contents Detail