Solution structure of ZZZ3 ZZ domain in complex with histone H3 tail
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS13:SG | 3:ZN1:ZN |
2 | na | sing | 1:CYS16:SG | 3:ZN1:ZN |
3 | na | sing | 1:CYS40:SG | 3:ZN1:ZN |
4 | na | sing | 1:CYS43:SG | 3:ZN1:ZN |
5 | na | sing | 1:CYS28:SG | 3:ZN1:ZN |
6 | na | sing | 1:CYS31:SG | 3:ZN1:ZN |
7 | na | sing | 1:HIS53:ND1 | 3:ZN1:ZN |
8 | na | sing | 1:HIS57:NE2 | 3:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 71.8 % (612 of 852) | 81.0 % (362 of 447) | 56.3 % (184 of 327) | 84.6 % (66 of 78) |
Backbone | 71.5 % (319 of 446) | 87.6 % (134 of 153) | 56.3 % (125 of 222) | 84.5 % (60 of 71) |
Sidechain | 74.2 % (353 of 476) | 77.9 % (229 of 294) | 67.4 % (118 of 175) | 85.7 % (6 of 7) |
Aromatic | 38.3 % (23 of 60) | 70.0 % (21 of 30) | 3.4 % (1 of 29) | 100.0 % (1 of 1) |
Methyl | 85.7 % (48 of 56) | 89.3 % (25 of 28) | 82.1 % (23 of 28) |
1. entity 1
GPLGSVQHVG FKCDNCGIEP IQGVRWHCQD CPPEMSLDFC DSCSDCLHET DIHKEDHQLE PIYR2. entity 2
ARTKQTARKS TGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM [U-13C; U-15N] ZZ domain of ZZZ3, 10 mM Histone H3, 100 mM sodium chloride, 20 mM TRIS, 7 % [U-99% 2H] D2O, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ZZ domain of ZZZ3 | [U-13C; U-15N] | 2 mM | |
2 | Histone H3 | natural abundance | 10 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | TRIS | natural abundance | 20 mM | |
5 | D2O | [U-99% 2H] | 7 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 4 mM [U-13C; U-15N] ZZ domain of ZZZ3, 100 mM sodium chloride, 20 mM TRIS, 7 % [U-99% 2H] D2O, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | ZZ domain of ZZZ3 | [U-13C; U-15N] | 4 mM | |
7 | sodium chloride | natural abundance | 100 mM | |
8 | TRIS | natural abundance | 20 mM | |
9 | D2O | [U-99% 2H] | 7 % |