Solution NMR Structure of a Class I Hydrophobin from Wallemia ichthyophaga
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS11:SG | 1:CYS70:SG |
2 | disulfide | sing | 1:CYS18:SG | 1:CYS64:SG |
3 | disulfide | sing | 1:CYS19:SG | 1:CYS49:SG |
4 | disulfide | sing | 1:CYS71:SG | 1:CYS84:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.0 % (912 of 960) | 92.9 % (459 of 494) | 98.4 % (365 of 371) | 92.6 % (88 of 95) |
Backbone | 96.1 % (511 of 532) | 95.7 % (178 of 186) | 97.7 % (254 of 260) | 91.9 % (79 of 86) |
Sidechain | 94.5 % (480 of 508) | 91.2 % (281 of 308) | 99.5 % (190 of 191) | 100.0 % (9 of 9) |
Aromatic | 100.0 % (16 of 16) | 100.0 % (8 of 8) | 100.0 % (7 of 7) | 100.0 % (1 of 1) |
Methyl | 100.0 % (108 of 108) | 100.0 % (54 of 54) | 100.0 % (54 of 54) |
1. entity 1
GSGWESKTGS CNTGKLACCD TNKKVQKSTG EESGLLHTGD VLDQVAIQCT QIPLLIGIAI EDECKNTPTC CEDVEDDGLV GINCTPIPLISolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5, Details 250 uM [U-13C; U-15N] WI1, 20 mM MES, 50 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | WI1 | [U-13C; U-15N] | 250 uM | |
2 | MES | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5, Details 100 uM [U-13C; U-15N] WI1, 20 mM MES, 50 mM sodium chloride, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | WI1 | [U-13C; U-15N] | 100 uM | |
5 | MES | natural abundance | 20 mM | |
6 | sodium chloride | natural abundance | 50 mM |