Solution structure of HIV-1 TAR with Tat RNA Binding Domain
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 61.5 % (431 of 701) | 77.1 % (299 of 388) | 43.3 % (120 of 277) | 33.3 % (12 of 36) |
Backbone | 52.8 % (228 of 432) | 69.0 % (149 of 216) | 39.2 % (78 of 199) | 5.9 % (1 of 17) |
Sidechain | 75.0 % (213 of 284) | 87.2 % (150 of 172) | 55.9 % (52 of 93) | 57.9 % (11 of 19) |
Aromatic | 69.4 % (100 of 144) | 91.7 % (66 of 72) | 41.8 % (23 of 55) | 64.7 % (11 of 17) |
Methyl | 50.0 % (3 of 6) | 66.7 % (2 of 3) | 33.3 % (1 of 3) |
1. entity 1
GGGCAGAUUG AGCCUGGGAG CUCUCUGCCC2. entity 2
GISYGRKKRR QRRRAHQSolvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM TAR RNA with deuterated NTPs in a combo of either AC, GU, or GA, 0.5 mM Tat RNA Binding Domain, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TAR RNA | natural abundance | 0.5 (±0.1) mM | |
2 | Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
3 | NaCl | natural abundance | 10 mM | |
4 | NaCl | natural abundance | 70 mM | |
5 | EDTA | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM [U-13C; U-15N] TAR RNA, 0.5 mM Tat RNA Binding Domain, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | TAR RNA | [U-13C; U-15N] | 0.5 (±0.1) mM | |
7 | Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
8 | NaCl | natural abundance | 10 mM | |
9 | NaCl | natural abundance | 70 mM | |
10 | EDTA | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM TAR RNA deuterated NTPs in a combo of either AC, GU, or GA, 0.5 mM Tat RNA Binding Domain, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | TAR RNA | natural abundance | 0.5 (±0.1) mM | |
12 | Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
13 | NaCl | natural abundance | 10 mM | |
14 | NaCl | natural abundance | 70 mM | |
15 | EDTA | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM TAR RNA, 0.5 mM [U-13C; U-15N] Tat RNA Binding Domain selectively labeled in each of the peptides: 1) R49, K50, R57, A58 2) K51, R52, Q54,R55 3) R53, Q54, R56, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | TAR RNA | natural abundance | 0.5 (±0.1) mM | |
17 | Tat RNA Binding Domain | [U-13C; U-15N] | 0.5 (±0.1) mM | |
18 | NaCl | natural abundance | 10 mM | |
19 | NaCl | natural abundance | 70 mM | |
20 | EDTA | natural abundance | 0.1 mM |