Solution structure of 7SK stem-loop 1 with HIV-1 Tat RNA Binding Domain
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.8 % (493 of 1100) | 52.8 % (320 of 606) | 30.2 % (134 of 443) | 76.5 % (39 of 51) |
Backbone | 22.6 % (160 of 707) | 24.6 % (90 of 366) | 18.5 % (60 of 324) | 58.8 % (10 of 17) |
Sidechain | 84.1 % (343 of 408) | 95.8 % (230 of 240) | 62.7 % (84 of 134) | 85.3 % (29 of 34) |
Aromatic | 80.5 % (206 of 256) | 96.1 % (123 of 128) | 57.3 % (55 of 96) | 87.5 % (28 of 32) |
Methyl | 50.0 % (3 of 6) | 66.7 % (2 of 3) | 33.3 % (1 of 3) |
1. entity 1
GGGAUCUGUC ACCCCAUUGA UCGCCGAGAG GCUGAUCUGG XUGGXUAGGC GGGUCCC2. entity 2
GISYGRKKRR QRRRAHQSolvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM 7SK Stem-loop 1 RNA, 0.5 mM [U-13C; U-15N] HIV-1 Tat RNA Binding Domain selectively labeled with the following schema: 1) R49, K50, R57, A58 2) K51, R52, Q54, R55 3) R53, Q54, R56, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | 7SK Stem-loop 1 RNA | natural abundance | 0.5 (±0.1) mM | |
2 | HIV-1 Tat RNA Binding Domain | [U-13C; U-15N] | 0.5 (±0.1) mM | |
3 | phosphates | natural abundance | 10 mM | |
4 | NaCl | natural abundance | 70 mM |
Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM 7SK Stem-loop 1 RNA, 0.5 mM HIV-1 Tat RNA Binding Domain, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | 7SK Stem-loop 1 RNA | natural abundance | 0.5 (±0.1) mM | |
6 | HIV-1 Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
7 | phosphates | natural abundance | 10 mM | |
8 | NaCl | natural abundance | 70 mM |
Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM 7SK Stem-loop 1 RNA, 0.5 mM [U-13C; U-15N] HIV-1 Tat RNA Binding Domain selectively labeled with the following schema: 1) R49, K50, R57, A58 2) K51, R52, Q54, R55 3) R53, Q54, R56, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | 7SK Stem-loop 1 RNA | natural abundance | 0.5 (±0.1) mM | |
10 | HIV-1 Tat RNA Binding Domain | [U-13C; U-15N] | 0.5 (±0.1) mM | |
11 | phosphates | natural abundance | 10 mM | |
12 | NaCl | natural abundance | 70 mM |
Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM [U-13C; U-15N] 7SK Stem-loop 1 RNA, 0.5 mM HIV-1 Tat RNA Binding Domain, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | 7SK Stem-loop 1 RNA | [U-13C; U-15N] | 0.5 (±0.1) mM | |
14 | HIV-1 Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
15 | phosphates | natural abundance | 10 mM | |
16 | NaCl | natural abundance | 70 mM |
Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM [U-13C; U-15N] 7SK Stem-loop 1 RNA, 0.5 mM HIV-1 Tat RNA Binding Domain, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | 7SK Stem-loop 1 RNA | [U-13C; U-15N] | 0.5 (±0.1) mM | |
18 | HIV-1 Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
19 | phosphates | natural abundance | 10 mM | |
20 | NaCl | natural abundance | 70 mM |
Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.4, Details 0.5 mM 7SK Stem-loop 1 RNA, 0.5 mM HIV-1 Tat RNA Binding Domain, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | 7SK Stem-loop 1 RNA | natural abundance | 0.5 (±0.1) mM | |
22 | HIV-1 Tat RNA Binding Domain | natural abundance | 0.5 (±0.1) mM | |
23 | phosphates | natural abundance | 10 mM | |
24 | NaCl | natural abundance | 70 mM |