BMRB: 30512

Solution structure of 7SK stem-loop 1

Authors

Pham, V.V., D'Souza, V.M.

Assembly

7SK RNA

Entity

1. 7SK RNA (polymer, Thiol state: not present), 57 monomers, 18421.88 Da Detail

GGGAUCUGUC ACCCCAUUGA UCGCCGAGAG GCUGAUCUGG XUGGXUAGGC GGGUCCC

Formula weight

18421.88 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 31.3 %, Completeness (bb): 10.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	31.3 % (275 of 879)	41.3 % (199 of 482)	13.4 % (49 of 365)	84.4 % (27 of 32)
Suger, PO4	10.7 % (65 of 605)	17.0 % (56 of 330)	3.3 % (9 of 275)
Nucleobase	76.6 % (210 of 274)	94.1 % (143 of 152)	44.4 % (40 of 90)	84.4 % (27 of 32)
Aromatic	75.4 % (184 of 244)	95.9 % (117 of 122)	44.4 % (40 of 90)	84.4 % (27 of 32)

1. entity 1

GGGAUCUGUC ACCCCAUUGA UCGCCGAGAG GCUGAUCUGG XUGGXUAGGC GGGUCCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 283 K, pH 5.4, Details 0.5 mM 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 90%H2O/10%D2O

#	Name	Isotope labeling	Type	Concentration
1	7SK RNA	natural abundance		0.5 (±0.1) mM
2	NaCl	natural abundance		10 mM
3	NaCl	natural abundance		70 mM
4	EDTA	natural abundance		0.1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 283 K, pH 5.4, Details 0.5 mM [U-13C; U-15N] 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 90%H2O/10%D2O

#	Name	Isotope labeling	Type	Concentration
5	7SK RNA	[U-13C; U-15N]		0.5 (±0.1) mM
6	NaCl	natural abundance		10 mM
7	NaCl	natural abundance		70 mM
8	EDTA	natural abundance		0.1 mM

Sample #3

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 283 K, pH 5.4, Details 0.5 mM [U-13C; U-15N] 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	7SK RNA	[U-13C; U-15N]		0.5 (±0.1) mM
10	NaCl	natural abundance		10 mM
11	NaCl	natural abundance		70 mM
12	EDTA	natural abundance		0.1 mM

Sample #4

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 283 K, pH 5.4, Details 0.5 mM 7SK RNA, 10mM Phosphate, 70mM NaCl, 0.1mM EDTA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
13	7SK RNA	natural abundance		0.5 (±0.1) mM
14	NaCl	natural abundance		10 mM
15	NaCl	natural abundance		70 mM
16	EDTA	natural abundance		0.1 mM

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Release date

2018-09-09

Citation

HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry
Pham, V.V., Salguero, C., Khan, S.N., Meagher, J., Brown, W., Humbert, N., de Rocquigny, H., Smith, J., D'Souza, V.M.
Nat. Commun. (2018), 9, 4266-4266, PubMed 30323330 , DOI 10.1038/s41467-018-06591-6 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. 7SK RNA, : 1 : 1 : 4 : 3 entities Detail

Experiments performed 4 experiments Detail