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Found 1 Document (1.034 seconds)

BMRB: 30517

6MIE

Solution NMR structure of the KCNQ1 voltage-sensing domain

Authors

Taylor, K.C., Kuenze, G., Smith, J.A., Meiler, J., McFeeters, R.L., Sanders, C.R.

Assembly

Potassium voltage-gated channel subfamily KQT member 1

Entity

1. Potassium voltage-gated channel subfamily KQT member 1 (polymer), 159 monomers, 18158.40 Da Detail

MGHHHHHHGV LARTHVQGRV YNFLERPTGW KCFVYHFAVF LIVLVCLIFS VLSTIEQYAA LATGTLFWME IVLVVFFGTE YVVRLWSAGC RSKYVGLWGR LRFARKPISI IDLIVVVASM VVLCVGSKGQ VFATSAIRGI RFLQILRMLH VDRQGGTWR

Formula weight

18158.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 73.5 %, Completeness (bb): 86.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.5 % (1413 of 1922)	75.5 % (738 of 978)	67.9 % (527 of 776)	88.1 % (148 of 168)
Backbone	86.1 % (818 of 950)	88.2 % (291 of 330)	83.2 % (385 of 463)	90.4 % (142 of 157)
Sidechain	63.6 % (710 of 1117)	68.7 % (445 of 648)	56.6 % (259 of 458)	54.5 % (6 of 11)
Aromatic	23.2 % (57 of 246)	37.4 % (46 of 123)	5.1 % (6 of 118)	100.0 % (5 of 5)
Methyl	87.3 % (206 of 236)	89.8 % (106 of 118)	84.7 % (100 of 118)

1. entity 1

MGHHHHHHGV LARTHVQGRV YNFLERPTGW KCFVYHFAVF LIVLVCLIFS VLSTIEQYAA LATGTLFWME IVLVVFFGTE YVVRLWSAGC RSKYVGLWGR LRFARKPISI IDLIVVVASM VVLCVGSKGQ VFATSAIRGI RFLQILRMLH VDRQGGTWR

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 13C; U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
7	MES	natural abundance	50 mM
8	EDTA	natural abundance	0.5 mM
9	TCEP	natural abundance	2 mM
10	LMPG	natural abundance	4 % w/v

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 13C; U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v Deuterated LMPG, 100% D2O

#	Name	Isotope labeling	Concentration
11	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
12	MES	natural abundance	50 mM
13	EDTA	natural abundance	0.5 mM
14	TCEP	natural abundance	2 mM
15	Deuterated LMPG	natural abundance	4 % w/v

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
16	KCNQ1-VSD	[U-100% 15N]	0.4 mM
17	MES	natural abundance	50 mM
18	EDTA	natural abundance	0.5 mM
19	TCEP	natural abundance	2 mM
20	LMPG	natural abundance	4 % w/v

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 mM LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
21	KCNQ1-VSD	[U-100% 15N]	0.2 mM
22	MES	natural abundance	50 mM
23	EDTA	natural abundance	0.5 mM
24	TCEP	natural abundance	2 mM
25	LMPG	natural abundance	4 mM

Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 mM LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
26	KCNQ1-VSD	[U-100% 15N]	0.2 mM
27	MES	natural abundance	50 mM
28	EDTA	natural abundance	0.5 mM
29	TCEP	natural abundance	2 mM
30	LMPG	natural abundance	4 mM

Sample #7

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	KCNQ1-VSD	[U-100% 15N]	0.2 mM
32	MES	natural abundance	50 mM
33	EDTA	natural abundance	0.5 mM
34	TCEP	natural abundance	2 mM
35	LMPG	natural abundance	4 % w/v

Sample #8

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
36	KCNQ1-VSD	[U-100% 15N]	0.2 mM
37	MES	natural abundance	50 mM
38	EDTA	natural abundance	0.5 mM
39	TCEP	natural abundance	2 mM
40	LMPG	natural abundance	4 % w/v

Sample #9

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
41	KCNQ1-VSD	[U-100% 15N]	0.2 mM
42	MES	natural abundance	50 mM
43	EDTA	natural abundance	0.5 mM
44	TCEP	natural abundance	2 mM
45	LMPG	natural abundance	4 % w/v

Sample #10

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
46	KCNQ1-VSD	[U-100% 15N]	0.2 mM
47	MES	natural abundance	50 mM
48	EDTA	natural abundance	0.5 mM
49	TCEP	natural abundance	2 mM
50	LMPG	natural abundance	4 % w/v

Sample #11

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
51	KCNQ1-VSD	[U-100% 15N]	0.2 mM
52	MES	natural abundance	50 mM
53	EDTA	natural abundance	0.5 mM
54	TCEP	natural abundance	2 mM
55	LMPG	natural abundance	4 % w/v

Sample #12

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
56	KCNQ1-VSD	[U-100% 15N]	0.2 mM
57	MES	natural abundance	50 mM
58	EDTA	natural abundance	0.5 mM
59	TCEP	natural abundance	2 mM
60	LMPG	natural abundance	4 % w/v

Sample #13

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
61	KCNQ1-VSD	[U-100% 15N]	0.2 mM
62	MES	natural abundance	50 mM
63	EDTA	natural abundance	0.5 mM
64	TCEP	natural abundance	2 mM
65	LMPG	natural abundance	4 % w/v

Protein Blocks Logo

Calculated from 10 models in PDB: 6MIE, Strand ID: A Detail

Release date

2018-10-02

Citation

Structure and physiological function of the human KCNQ1 channel voltage sensor intermediate state
Taylor, K.C., Kang, P.W., Hou, P., Kuenze, G., Yang, N., Smith, J.A., Shi, J., Huang, H., White, K.M., Peng, D., George, A.L., Meiler, J., McFeeters, R.L., Cui, J., Sanders, C.R.
Elife (2020), 9, e53901-e53901, PubMed 32096762 , DOI 10.7554/eLife.53901 , Abstract

Related entities 1. Potassium voltage-gated channel subfamily KQT member 1, : 1 : 5 : 100 entities Detail

Interaction partners 1. Potassium voltage-gated channel subfamily KQT member 1, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 24 experiments Detail

1 3D HNCA

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

2 3D HNCO

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

3 3D HN(CO)CA

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

4 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

5 3D HNCACB

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

6 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
1	KCNQ1-VSD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	0.5 mM
4	TCEP	natural abundance	2 mM
5	LMPG	natural abundance	4 % w/v

7 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz CPQCI probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 13C; U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
7	MES	natural abundance	50 mM
8	EDTA	natural abundance	0.5 mM
9	TCEP	natural abundance	2 mM
10	LMPG	natural abundance	4 % w/v

8 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz CPQCI probe

Sample

#	Name	Isotope labeling	Concentration
6	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
7	MES	natural abundance	50 mM
8	EDTA	natural abundance	0.5 mM
9	TCEP	natural abundance	2 mM
10	LMPG	natural abundance	4 % w/v

9 3D C(CO)NH

Instrument

Bruker AvanceIII - 600 MHz CPQCI probe

Sample

#	Name	Isotope labeling	Concentration
6	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
7	MES	natural abundance	50 mM
8	EDTA	natural abundance	0.5 mM
9	TCEP	natural abundance	2 mM
10	LMPG	natural abundance	4 % w/v

10 3D HCCH-COSY

Instrument

Bruker AvanceIII - 600 MHz CPQCI probe

Sample

#	Name	Isotope labeling	Concentration
6	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
7	MES	natural abundance	50 mM
8	EDTA	natural abundance	0.5 mM
9	TCEP	natural abundance	2 mM
10	LMPG	natural abundance	4 % w/v

11 2D 1H-13C HSQC aliphatic

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 13C; U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v Deuterated LMPG, 100% D2O

#	Name	Isotope labeling	Concentration
11	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
12	MES	natural abundance	50 mM
13	EDTA	natural abundance	0.5 mM
14	TCEP	natural abundance	2 mM
15	Deuterated LMPG	natural abundance	4 % w/v

12 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
11	KCNQ1-VSD	[U-100% 13C; U-100% 15N]	0.4 mM
12	MES	natural abundance	50 mM
13	EDTA	natural abundance	0.5 mM
14	TCEP	natural abundance	2 mM
15	Deuterated LMPG	natural abundance	4 % w/v

13 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.4 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 % w/v LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
16	KCNQ1-VSD	[U-100% 15N]	0.4 mM
17	MES	natural abundance	50 mM
18	EDTA	natural abundance	0.5 mM
19	TCEP	natural abundance	2 mM
20	LMPG	natural abundance	4 % w/v

14 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
16	KCNQ1-VSD	[U-100% 15N]	0.4 mM
17	MES	natural abundance	50 mM
18	EDTA	natural abundance	0.5 mM
19	TCEP	natural abundance	2 mM
20	LMPG	natural abundance	4 % w/v

15 2D IPAP-HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
16	KCNQ1-VSD	[U-100% 15N]	0.4 mM
17	MES	natural abundance	50 mM
18	EDTA	natural abundance	0.5 mM
19	TCEP	natural abundance	2 mM
20	LMPG	natural abundance	4 % w/v

16 2D IPAP-HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 mM LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
21	KCNQ1-VSD	[U-100% 15N]	0.2 mM
22	MES	natural abundance	50 mM
23	EDTA	natural abundance	0.5 mM
24	TCEP	natural abundance	2 mM
25	LMPG	natural abundance	4 mM

17 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.2 mM [U-100% 15N] KCNQ1-VSD, 50 mM MES, 0.5 mM EDTA, 2 mM TCEP, 4 mM LMPG, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
26	KCNQ1-VSD	[U-100% 15N]	0.2 mM
27	MES	natural abundance	50 mM
28	EDTA	natural abundance	0.5 mM
29	TCEP	natural abundance	2 mM
30	LMPG	natural abundance	4 mM

18 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
31	KCNQ1-VSD	[U-100% 15N]	0.2 mM
32	MES	natural abundance	50 mM
33	EDTA	natural abundance	0.5 mM
34	TCEP	natural abundance	2 mM
35	LMPG	natural abundance	4 % w/v

19 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
36	KCNQ1-VSD	[U-100% 15N]	0.2 mM
37	MES	natural abundance	50 mM
38	EDTA	natural abundance	0.5 mM
39	TCEP	natural abundance	2 mM
40	LMPG	natural abundance	4 % w/v

20 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
41	KCNQ1-VSD	[U-100% 15N]	0.2 mM
42	MES	natural abundance	50 mM
43	EDTA	natural abundance	0.5 mM
44	TCEP	natural abundance	2 mM
45	LMPG	natural abundance	4 % w/v

21 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
46	KCNQ1-VSD	[U-100% 15N]	0.2 mM
47	MES	natural abundance	50 mM
48	EDTA	natural abundance	0.5 mM
49	TCEP	natural abundance	2 mM
50	LMPG	natural abundance	4 % w/v

22 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
51	KCNQ1-VSD	[U-100% 15N]	0.2 mM
52	MES	natural abundance	50 mM
53	EDTA	natural abundance	0.5 mM
54	TCEP	natural abundance	2 mM
55	LMPG	natural abundance	4 % w/v

23 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
56	KCNQ1-VSD	[U-100% 15N]	0.2 mM
57	MES	natural abundance	50 mM
58	EDTA	natural abundance	0.5 mM
59	TCEP	natural abundance	2 mM
60	LMPG	natural abundance	4 % w/v

24 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz CPTCI probe

Sample

#	Name	Isotope labeling	Concentration
61	KCNQ1-VSD	[U-100% 15N]	0.2 mM
62	MES	natural abundance	50 mM
63	EDTA	natural abundance	0.5 mM
64	TCEP	natural abundance	2 mM
65	LMPG	natural abundance	4 % w/v

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30517_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30517_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1577		91.2 (chain: 1, length: 159)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 159, Sequence coverage: 91.2%
- Total number of assignments
  - ¹H chemical shifts: 926
  - ¹³C chemical shifts: 506
  - ¹⁵N chemical shifts: 145
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
141	ARG	HH11	6.624
141	ARG	HH12	6.624
141	ARG	HH21	6.576
141	ARG	HH22	6.576

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	978	710	72.6
¹³C chemical shifts	776	506	65.2
¹⁵N chemical shifts	181	145	80.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	330	282	85.5
¹³C chemical shifts	318	263	82.7
¹⁵N chemical shifts	157	139	88.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	648	428	66.0
¹³C chemical shifts	458	243	53.1
¹⁵N chemical shifts	24	6	25.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	122	106	86.9
¹³C chemical shifts	122	98	80.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	123	41	33.3
¹³C chemical shifts	118	0	0.0
¹⁵N chemical shifts	5	5	100.0

Keywords KCNQ1, Kv7.1, MEMBRANE PROTEIN, ion channel, potassium channel, voltage sensor

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Found 1 Document (1.034 seconds)

BMRB: 30517

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Sample #12

Sample #13

Related entities 1. Potassium voltage-gated channel subfamily KQT member 1, : 1 : 5 : 100 entities Detail

Interaction partners 1. Potassium voltage-gated channel subfamily KQT member 1, : 2 interactors Detail

Experiments performed 24 experiments Detail

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